| 陈 娟,尤卞红.高危人乳头状瘤病毒感染宫颈癌患者miR-34a-5p表达及意义[J].肿瘤学杂志,2020,26(11):977-980. |
| 高危人乳头状瘤病毒感染宫颈癌患者miR-34a-5p表达及意义 |
| Expression of MicroRNA-34a-5p in Cervical Cancer Patients with High Risk of Human Papillomavirus Infection and Its Clinical Significance |
| 投稿时间：2020-03-17 |
| DOI：10.11735/j.issn.1671-170X.2020.11.B010 |
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| 中文关键词: 宫颈癌 乳头状瘤病毒 miR-34a-5p 血清 |
| 英文关键词:cervical cancer papilloma virus miR-34a-5p serum |
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| 中文摘要: |
| 摘 要：［目的］ 探讨高危人乳头状瘤病毒（high risk human papillomavirus，HR-HPV）感染宫颈癌患者组织及血清miR-34a-5p的表达以及血清miR-34a-5p对宫颈癌的诊断价值。［方法］ 采用RT-PCR检测70例HR-HPV阳性宫颈癌组织和癌旁组织、100例HR-HPV阳性宫颈癌患者血清、80例HR-HPV阳性宫颈上皮内瘤病变(CIN)患者血清、80例健康体检者血清中miR-34a-5p的表达水平。Pearson法分析组织和血清中miR-34a-5p表达量的相关性，分析miR-34a-5p与宫颈癌临床病理特征的关系，分析血清miR-34a-5p诊断宫颈癌的价值。［结果］ 宫颈癌组织miR-34a-5p相对表达量明显低于癌旁组织（P＜0.05）。宫颈癌患者血清miR-34a-5p相对表达量明显低于CIN患者和正常人（P＜0.05），CIN患者血清miR-34a-5p相对表达量明显低于正常人（P＜0.05）。癌组织miR-34a-5p相对表达量与淋巴结转移有关(P<0.05)。血清miR-34a-5p相对表达量与淋巴结转移和远处转移有关(P<0.05)。miR-34a-5p在癌组织与血清中的相对表达量呈正相关（r=0.459，P＜0.05）。血清miR-34a-5p诊断宫颈癌的ROC曲线下面积为0.860（95%CI：0.803~0.917）。［结论］ HR-HPV宫颈癌患者血清miR-34a-5p下调，与癌组织miR-34a-5p表达呈正相关，有望成为宫颈癌诊断的分子标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of miR-34a-5p in tissues and serum of cervical cancer patients with high risk human papillomavirus(HR-HPV) infection and its clinical significance. ［Methods］ The expression of miR-34a-5p in cervical cancer tissues and adjacent tissues of 70 HR-HPV-positive patients was detected by RT-PCR. The expression of miR-34a-5p in serum samples of 100 HR-HPV-positive cervical cancer patients，80 HR-HPV positive cervical intraepithelial neoplasia(CIN) patients and 80 healthy subjects was also detected. Pearson correlation was used to analyze the correlation between the expression of miR-34a-5p in tissue and serum，and the relationship between miR-34a-5p and the clinicopathological characteristics of cervical cancer was also analyzed. ［Results］ The relative expression of miR-34a-5p in cervical cancer tissues was significantly lower than that in adjacent tissues(P<0.05). The relative expression of miR-34a-5p in serum samples of cervical cancer patients was significantly lower than that of CIN patients and normal subjects(P<0.05). The relative expression of serum miR-34a-5p in CIN patients was significantly lower than that in normal subjects(P<0.05). The relative expression of tissue miR-34a-5p was correlated with lymph node metastasis(P<0.05). The relative expression of serum miR-34a-5p was correlated with lymph node metastasis and distant metastasis(P<0.05). The relative expression of miR-34a-5p in cancer tissue and serum was positively correlated with each other(r=0.459，P<0.05). The area under ROC curve of serum miR-34a-5p was 0.860(95% CI：0.803 ~ 0.917). ［Conclusion］ The down-regulation of serum miR-34a-5p in HR-HPV positive cervical cancer patients is positively correlated with the expression of miR-34a-5p in cancer tissues，which is expected to be a molecular marker for diagnosis of cervical cancer. |
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