| 邰小华,冯联忠,章 波.MiR-1271靶向ZEB1抑制甲状腺乳头状癌细胞增殖和转移[J].肿瘤学杂志,2020,26(10):879-885. |
| MiR-1271靶向ZEB1抑制甲状腺乳头状癌细胞增殖和转移 |
| MiR-1271 Targets ZEB1 to Inhibit the Proliferation and Metastasis of Papillary Thyroid Carcinoma Cells |
| 投稿时间：2019-11-12 |
| DOI：10.11735/j.issn.1671-170X.2020.10.B006 |
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| 中文关键词: 甲状腺乳头状癌 miR-1271 ZEB1 增殖 转移 |
| 英文关键词:papillary thyroid carcinoma miR-1271 ZEB1 proliferation metastasis |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-1271对甲状腺乳头状癌细胞增殖和转移的影响及其作用机制。［方法］ 收集20例手术切除甲状腺乳头状癌患者的癌组织和对应的癌旁组织标本。采用RT-qPCR检测组织和细胞系中miR-1271和ZEB1的表达水平；CCK-8检测K1细胞增殖活力；Transwell检测K1细胞侵袭和迁移能力；Western blot检测蛋白的表达水平；双荧光素酶报告基因验证miR-1271与ZEB1的靶向关系。［结果］ miR-1271在甲状腺乳头状癌组织中的表达水平低于癌旁组织组织（1.175±0.534 vs 3.918±0.512，P<0.001)。与人甲状腺滤泡上皮正常细胞（0.985±0.062）相比，miR-1271在癌细胞系（TPC-1：0.752±0.052，K1：0.318±0.042，BCPAP：0.584±0.045）中的表达水平明显下调(P均<0.05)，且在K1细胞中表达最低（P=0.016）。双荧光素酶报告基因结果显示，miR-1271通过结合ZEB1基因的3′UTR，进而抑制其表达水平。过表达ZEB1可明显缓解miR-1271对K1细胞增殖和转移的抑制作用。［结论］ miR-1271通过靶向下调ZEB1的表达，进而抑制K1细胞增殖、侵袭、迁移和EMT。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the effect of miR-1271 on the proliferation and metastasis of papillary thyroid carcinoma cells and its underlying mechanism.［Methods］ Twenty samples of cancer tissue and corresponding pericancerous tissues were collected from patients with papillary thyroid carcinoma at the Jiaxing Second Hospital from February 2016 to August 2018. RT-qPCR was used to detect the expression level of miR-1271 and ZEB1 in clinical samples and papillary thyroid carcinoma cell lines TPC-1，K1，BCPAP and normal human thyroid follicular epithelial Nthy-ori3-1 cells. The proliferation，invasion，and migration capacity of K1 cells were examined using CCK-8 assay and Transwell assay. Western blot was performed to measure protein level. Dual-luciferase reporter gene assay was applied to verify the interaction between miR-1271 and ZEB1 in papillary thyroid carcinoma. ［Results］ Expression of miR-1271 in the papillary thyroid carcinoma tissues was higher than that in the corresponding pericancerous tissues(1.175±0.534 vs 3.918±0.512，P<0.001). Compared with Nthy-ori3-1 cells(0.985±0.062)，the expression of miR-1271 was down-regulated in papillary thyroid carcinoma cell lines(TPC-1：0.752±0.052，K1：0.318±0.042，BCPAP：0.584±0.045，all P<0.05). Overexpression of miR-1271 significantly suppressed the proliferation，invasion，migration and epithelial-mesenchymal transition(EMT). Dual-luciferase reporter gene assay results showed that miR-1271 decreased the expression of ZEB1 by binding to its 3′-untranslated region(UTR). Upregulation of ZEB1 attenuated the inhibitory effect of miR-1271 on the proliferation and metastasis of K1 cells. ［Conclusion］ miR-1271 can inhibit the proliferation，invasion，migration and EMT in papillary thyroid carcinoma cells by down-regulation of ZEB1 expression. |
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