邰小华,冯联忠,章 波.MiR-1271靶向ZEB1抑制甲状腺乳头状癌细胞增殖和转移[J].肿瘤学杂志,2020,26(10):879-885.
MiR-1271靶向ZEB1抑制甲状腺乳头状癌细胞增殖和转移
MiR-1271 Targets ZEB1 to Inhibit the Proliferation and Metastasis of Papillary Thyroid Carcinoma Cells
投稿时间:2019-11-12  
DOI:10.11735/j.issn.1671-170X.2020.10.B006
中文关键词:  甲状腺乳头状癌  miR-1271  ZEB1  增殖  转移
英文关键词:papillary thyroid carcinoma  miR-1271  ZEB1  proliferation  metastasis
基金项目:
作者单位
邰小华 嘉兴市第二医院 
冯联忠 嘉兴市第二医院 
章 波 嘉兴市第二医院 
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中文摘要:
      摘 要:[目的] 探讨miR-1271对甲状腺乳头状癌细胞增殖和转移的影响及其作用机制。[方法] 收集20例手术切除甲状腺乳头状癌患者的癌组织和对应的癌旁组织标本。采用RT-qPCR检测组织和细胞系中miR-1271和ZEB1的表达水平;CCK-8检测K1细胞增殖活力;Transwell检测K1细胞侵袭和迁移能力;Western blot检测蛋白的表达水平;双荧光素酶报告基因验证miR-1271与ZEB1的靶向关系。[结果] miR-1271在甲状腺乳头状癌组织中的表达水平低于癌旁组织组织(1.175±0.534 vs 3.918±0.512,P<0.001)。与人甲状腺滤泡上皮正常细胞(0.985±0.062)相比,miR-1271在癌细胞系(TPC-1:0.752±0.052,K1:0.318±0.042,BCPAP:0.584±0.045)中的表达水平明显下调(P均<0.05),且在K1细胞中表达最低(P=0.016)。双荧光素酶报告基因结果显示,miR-1271通过结合ZEB1基因的3′UTR,进而抑制其表达水平。过表达ZEB1可明显缓解miR-1271对K1细胞增殖和转移的抑制作用。[结论] miR-1271通过靶向下调ZEB1的表达,进而抑制K1细胞增殖、侵袭、迁移和EMT。
英文摘要:
      Abstract:[Objective] To explore the effect of miR-1271 on the proliferation and metastasis of papillary thyroid carcinoma cells and its underlying mechanism.[Methods] Twenty samples of cancer tissue and corresponding pericancerous tissues were collected from patients with papillary thyroid carcinoma at the Jiaxing Second Hospital from February 2016 to August 2018. RT-qPCR was used to detect the expression level of miR-1271 and ZEB1 in clinical samples and papillary thyroid carcinoma cell lines TPC-1,K1,BCPAP and normal human thyroid follicular epithelial Nthy-ori3-1 cells. The proliferation,invasion,and migration capacity of K1 cells were examined using CCK-8 assay and Transwell assay. Western blot was performed to measure protein level. Dual-luciferase reporter gene assay was applied to verify the interaction between miR-1271 and ZEB1 in papillary thyroid carcinoma. [Results] Expression of miR-1271 in the papillary thyroid carcinoma tissues was higher than that in the corresponding pericancerous tissues(1.175±0.534 vs 3.918±0.512,P<0.001). Compared with Nthy-ori3-1 cells(0.985±0.062),the expression of miR-1271 was down-regulated in papillary thyroid carcinoma cell lines(TPC-1:0.752±0.052,K1:0.318±0.042,BCPAP:0.584±0.045,all P<0.05). Overexpression of miR-1271 significantly suppressed the proliferation,invasion,migration and epithelial-mesenchymal transition(EMT). Dual-luciferase reporter gene assay results showed that miR-1271 decreased the expression of ZEB1 by binding to its 3′-untranslated region(UTR). Upregulation of ZEB1 attenuated the inhibitory effect of miR-1271 on the proliferation and metastasis of K1 cells. [Conclusion] miR-1271 can inhibit the proliferation,invasion,migration and EMT in papillary thyroid carcinoma cells by down-regulation of ZEB1 expression.
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