| 王桂臣,薛 鸣,高 阳.RNA干扰NF-κBp65基因表达对胃癌细胞株SGC-790增殖、凋亡的影响[J].肿瘤学杂志,2020,26(8):679-683. |
| RNA干扰NF-κBp65基因表达对胃癌细胞株SGC-790增殖、凋亡的影响 |
| RNA Interference of NF-κBp65 on Proliferation and Apoptosis of Gastric Cancer SGC-790 Cells |
| 投稿时间：2020-03-03 |
| DOI：10.11735/j.issn.1671-170X.2020.08.B004 |
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| 中文关键词: 胃肿瘤 NF-κBp65 细胞增殖 STAT3信号通路 |
| 英文关键词:gastric cancer NF-κBp65 cell proliferation STAT3 signaling pathway |
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| 中文摘要: |
| 摘 要：［目的］探讨RNA干扰NF-κBp65基因表达对胃癌细胞株SGC-790增殖、凋亡的影响。［方法］ 培养SGC-790胃癌细胞株，分为阴性对照组、siRNA-NC组、NF-κBp65-siRNA转染组。检测不同组细胞增殖抑制率及凋亡率，采用Western blot检测STAT3信号通路相关蛋白表达水平。［结果］ （1）MTT检测发现，在48h及72h时，NF-κBp65-siRNA组的细胞抑制率显著高于空白组与siRNA-NC组（P均<0.05）。（2）流式细胞术检测发现，48h时，空白组、siRNA-NC组、NF-κBp65-siRNA组的细胞凋亡率分别为5.85%±0.25%、6.13%±0.39%、16.05%±0.14%，NF-κBp65-siRNA组的细胞凋亡率显著增加（P<0.001）。（3）Transwell检测结果显示，空白组、siRNA-NC组、NF-κBp65-siRNA组穿过Transwell小室的细胞数量分别为379.62±10.51、386.86±11.42、89.62±5.73，NF-κBp65-siRNA组的穿膜细胞数量显著少于空白组与siRNA-NC组（P均<0.001）。（4）Western-blot检测显示，与空白组相比，NF-κBp65-siRNA组中p-STAT3、PCNA、MMP-2蛋白的表达量均显著下降（P均<0.05）。［结论］在SGC-790胃癌细胞株中，抑制NF-κBp65表达水平可能通过降低STAT3信号通路活性，抑制细胞增殖并促进凋亡。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of RNA interference of NF-κBp65 on proliferation and apoptosis of gastric cancer cell line SGC-790. ［Methods］ SGC-790 gastric cancer cells were divided into control group，siRNA-NC group and NF-κBp65-siRNA group. Cell proliferation inhibition rate and apoptosis rate were determined with MTT assay and cytometry，respectively；cell migration was examined with Transwell test；and the expression of STAT3 signaling pathway related proteins were detected by Western blot. ［Results］ MTT assay showed that the inhibition rate of SGC-790 cells in NF-κBp65-siRNA group was significantly higher than that in control group and siRNA NC group at 48 h and 72 h(all P<0.05). Flow cytometry showed that the apoptosis rate in NF-κBp65-siRNA group was significantly higher than those in control group and siRNA-NC group(16.05%±0.14% vs 5.85%±0.25% and 6.13%±0.39%，P<0.001). The Transwell test results showed that the number of passing cells in NF-κBp65-siRNA group was significantly less than that in control group and siRNA-NC group(89.62±5.73 vs 379.62±10.51 and 386.86±11.42，P<0.001). Compared with the control group，the expression of p-STAT3，PCNA and MMP-2 protein in NF-κBp65-siRNA group decreased significantly(all P<0.05). ［Conclusion］ The knockdown of NF-κBp65 can inhibit cell proliferation and promote apoptosis in SGC-790 gastric cancer cells，which may be associated with down-regulation of STAT3 signaling pathway activity. |
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