| 裴大兵,张翼臻,苏 坚.RORα激动剂SR1078抑制人胃癌MGC803细胞增殖与迁移侵袭的作用[J].肿瘤学杂志,2020,26(8):674-678. |
| RORα激动剂SR1078抑制人胃癌MGC803细胞增殖与迁移侵袭的作用 |
| Effect of RORα Agonist SR1078 on Proliferation，Migration and Invasion in Human Gastric Cancer MGC803 Cells |
| 投稿时间：2019-07-31 |
| DOI：10.11735/j.issn.1671-170X.2020.08.B003 |
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| 中文关键词: RORα SR1078 人胃癌MGC803细胞 增殖 迁移 侵袭 |
| 英文关键词:RORα SR1078 human gastric cancer MGC803 cells proliferation migration invasion |
| 基金项目:国家自然科学基金(81374013；81641112) |
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| 中文摘要: |
| 摘 要：［目的］ 观察RORα激动剂SR1078对人胃癌MGC803细胞增殖、迁移与侵袭的影响。［方法］ 实验分为10μmol/L SR1078处理MGC803细胞组与MGC803细胞组。Western blot 与免疫荧光检测RORα表达，MTT检测细胞增殖；细胞划痕和Transwell实验分别检测细胞迁移与侵袭。［结果］ Western blot检测显示，10μmol/L SR1078处理组较对照组RORα蛋白明显升高，差异有统计学意义(P<0.05)。免疫荧光显示，SR1078处理MGC803细胞RORα表达增强。MTT检测显示，10μmol/L SR1078作用MGC803细胞24h、48h、72h、96h后，增殖抑制率分别为37.2%、46.9%、52.7%、68.6%，SR1078可呈时间依赖性抑制MGC803细胞增殖 (P<0.05)。划痕实验显示，10μmol/L SR1078处理组迁移距离(5.74±0.078μm)明显低于对照组 (9.37±0.098μm)，差异有统计学意义(P<0.05)。Transwell迁移实验显示，10μmol/L SR1078处理组迁移细胞(80±1.155个)较对照组(103±1.155个)明显减少，差异有统计学意义(P<0.05)。侵袭实验显示，10μmol/L SR1078处理组细胞穿膜细胞(19.67±1.764个)较MGC803细胞（40.67±1.202个）明显减少，差异有统计学意义(P<0.05)。［结论］SR1078可通过上调RORα抑制MGC803细胞增殖与迁移侵袭。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of RORα agonist SR1078 on the proliferation，migration and invasion of human gastric cancer MGC803 cells. ［Methods］ Human gastric cancer MGC803 cells were treated with 10 μmol/L SR1078. Western blot and immunofluorescence were used to detect RORα expression，and MTT was used to detect cell proliferation. Cell migration and invasion were determined by cell scratch and Transwell assay respectively. ［Results］ Western blotting showed that RORα protein was significantly increased in SR1078 treatment group compared with the control group(P<0.05). Immunofluorescence showed that RORα expression was enhanced in MGC803 cells treated with SR1078. MTT showed that the proliferation of MGC803 cells were inhibited by 37.2%，46.9%，52.7% and 68.6% after SR1078 treatment for 24h，48h，72h and 96h，respectively，and it was in a time-dependent manner(P<0.05). The scratching test showed that the migration distance of SR1078 treatment group was decreased ［（5.74±0.078）μm］ compared with ［(9.37±0.098) μm］ in the control group(P<0.05). Transwell assay showed that there were 80±1.1550 migration cells in SR1078 treatment group，compared with 103±1.155 cells in the control group(P<0.05). Invasion test showed that the number of invasion cells in SR1078 treatment group were significantly lower than that in the control group(P<0.05). ［Conclusion］ SR1078 can inhibit the proliferation， migration and invasion of MGC803 cells by up-regulating RORα expression. |
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