| 陈 冉,戴立玲,倪 静.SOX1对宫颈癌细胞增殖、迁移和侵袭的影响[J].肿瘤学杂志,2020,26(7):626-629. |
| SOX1对宫颈癌细胞增殖、迁移和侵袭的影响 |
| Effect of SOX1 on Proliferation，Migration and Invasion of Cervical Cancer Cells |
| 投稿时间：2019-10-22 |
| DOI：10.11735/j.issn.1671-170X.2020.07.B012 |
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| 中文关键词: 宫颈癌 性别决定区Y框蛋白1 增殖 迁移 侵袭 |
| 英文关键词:cervical cancer sex determining region Y-box protein 1 proliferation migration invasion |
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| 中文摘要: |
| 摘 要：［目的］ 探究性别决定区Y框蛋白1（sex determining region Y-box，SOX1）对宫颈癌细胞增殖、迁移和侵袭的影响。［方法］ 选择稳定表达SOX1基因的HeLa细胞株为实验组，同时选择稳定表达空白质粒的HeLa细胞株和HeLa细胞为对照组。采用二甲基偶氮唑蓝（MTT）法检测SOX1对HeLa细胞增殖的影响；通过细胞基质黏附实验、体外细胞迁移实验、体外细胞侵袭实验分别研究SOX1对HeLa细胞粘附、迁移、侵袭能力的影响；采用明胶酶谱法检测细胞内相关蛋白的水平。［结果］ 在研究第1天，两组的增殖能力比较差异无统计学意义（P>0.05），实验组在研究第3天（0.526±0.067）、第5天（1.169±0.148）、第7天（1.160±0.134）的增殖能力均显著性低于对照组（P<0.05）。实验组黏附率（0.754±0.041）显著性低于对照组（0.931±0.135）（P<0.05）；实验组在24h（5.84±1.20）、48h（10.28±3.01）、72h（14.51±2.31）迁移距离均显著性小于对照组（P<0.05）；实验组侵袭细胞的数量（120.09±10.04个）明显较对照组（215.67±6.98个）少（P<0.05）。实验组基质金属蛋白酶2（0.981±0.199）、基质金属蛋白酶9（0.536±0.033）、波形纤维蛋白（0.429±0.029）表达水平显著性低于对照组，SOX1、E钙黏附蛋白水平明显高于对照组（P<0.05）。［结论］ SOX1表达水平升高,会使宫颈癌细胞的增殖、迁移和侵袭抑制，MMP2、MMP9、Vimentin蛋白表达水平降低，E-cadherin表达水平升高。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of sex determining region Y-box(SOX1) on the proliferation，migration and invasion of cervical cancer cells. ［Methods］ HeLa cell line stably expressing SOX1 gene was selected as the study group，while HeLa cell line stably expressing blank plasmid and HeLa cell line as the control group. The effect of SOX1 on HeLa cell proliferation was detected by MTT method， the effect of SOX1 on HeLa cell adhesion，migration and invasion was studied by cell matrix adhesion test，cell migration test and cell invasion test，the level of related proteins in HeLa cell was detected by gelatin zymogram. ［Results］ There was no significant difference in proliferation rate at d1 of cell culture between study group and control groups(P>0.05). The proliferation rate on d3(0.526±0.067)，d5(1.169±0.148) and d7(1.160±0.134) in study group was lower than that in control group(P<0.05). The adhesion rate in study group was significantly lower than that in control group(0.754±0.041 vs 0.931±0.135，P<0.05)，the migration distance of the study group at 24h(5.84±1.20)，48h(10.28±3.01)，72h(14.51±2.31) was significantly shorter than that of control group(P<0.05)， the number of invasive cells in the study group was significantly less than that of control group(120.09±10.04 vs 215.67±6.98，P<0.05). The expression levels of matrix metalloproteinase-2(MMP2)(0.981±0.199)，matrix metalloproteinase-9(MMP9)(0.536±0.033)，vimentin(0.429±0.029)， and the levels of SOX1 and E-cadherin in the study group were significantly lower than those in control group(P<0.05). ［Conclusion］ High SOX1 expression can inhibit the proliferation，migration and invasion of cervical cancer cells，and can decrease the expression of MMP2，MMP9 and vimentin，and increase the expression of E-cadherin. |
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