| 唐小乔,桑剑锋,张 寅.CD147在乳腺癌细胞中的表达及对癌细胞增殖及TGF-β信号通路的影响[J].肿瘤学杂志,2020,26(5):413-417. |
| CD147在乳腺癌细胞中的表达及对癌细胞增殖及TGF-β信号通路的影响 |
| Expression of CD147 in Breast Cancer Cells and Its Effect on Proliferation and TGF-β Signaling |
| 投稿时间：2019-10-30 |
| DOI：10.11735/j.issn.1671-170X.2020.05.B009 |
|
 |
| 中文关键词: 细胞外基质金属蛋白酶诱导因子 乳腺癌 增殖 转化生长因子-β |
| 英文关键词:extracellular matrix metalloproteinase inducer breast cancer proliferation transforming growth factor-β. |
| 基金项目:南京市医学科技发展项目（YKK17063） |
|
| 摘要点击次数: 1119 |
| 全文下载次数: 200 |
| 中文摘要: |
| 摘 要：［目的］ 观察细胞外基质金属蛋白酶诱导因子(CD147)在乳腺癌细胞表达及对癌细胞增殖及转化生长因子-β（TGF-β）信号通路的影响。［方法］ 收集2018年4月至2019年2月我院诊治的50例乳腺癌患者的乳腺癌和癌旁组织样本，CD147表达采用免疫组化检测。采用Western blot检测不同乳腺癌细胞中CD147蛋白表达，乳腺癌细胞HCC1806中转染siRNA后检测乳腺癌细胞增殖能力和TGF-β信号通路蛋白表达。［结果］ 与癌旁组织相比，乳腺癌组织中CD147蛋白表达明显上调（χ2=54.958，P<0.001）。与正常乳腺上皮细胞MCF-10A相比，乳腺癌细胞中CD147蛋白表达水平明显上调（F=10.841，P<0.001）。与对照组相比，转染CD147-siRNA后第2d和3d细胞增殖能力明显下降（t=5.029和5.316，P=0.007和0.006），同时TGF-β、磷酸化SMAD家族成员3（p-Smad3）和转录因子E2F（E2F4）蛋白水平明显升高（t=7.934，10.427和9.985，P=0.001，<0.001和0.001），而Myc家族蛋白c（c-Myc）蛋白水平明显下降（t=10.137，P=0.001）。［结论］ 乳腺癌中CD147表达明显上调，CD147可能通过抑制TGF-β/Smad3信号通路来促进乳腺癌细胞增殖。乳腺癌中CD147表达在乳腺癌诊断和治疗中具有一定临床意义。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of CD147 in breast cancer cells and its effect on proliferation and TGF-β signaling. ［Methods］ The expression of CD147 in breast cancer and pericancerous tissue samples of 50 breast cancer patients treated in our hospital from April 2018 to February 2019 was detected by immunohistochemistry. Western blotting was used to detect CD147 protein expression in breast cancer cell lines. Breast cancer HCC1806 cells were transfected with siRNA，the cell proliferation capacity and the expression of TGF-β signaling pathway protein were detected. ［Results］ Compared with the adjacent tissues，CD147 protein expression in breast cancer tissues was significantly up-regulated(χ2=54.958，P<0.001）. Compared with MCF-10A normal breast epithelial cells，CD147 protein expression was significantly up-regulated in HCC1806 breast cancer cells（F=10.841，P<0.001）. Compared with the control group，the proliferation of CD147-siRNA transfected HCC1806 cells significantly decreased cells at d2 and d3 after transfection（t=5.029 and 5.316，P=0.007 and 0.006），the expression levels of TGF-β，p-Smad3 and E2F4 proteins were significantly increased(t=7.934，10.427 and 9.985，P=0.001，<0.001 and 0.001），while c-Myc protein levels were significantly decreased(t=10.137，P=0.001）. ［Conclusion］ CD147 expression is significantly up-regulated in breast cancer，which may promote proliferation of breast cancer cells by inhibiting TGF-β /Smad3 signaling pathway. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |