| 王清富,朱立新,秦爱萍.溶血磷脂酸通过调控PI3K/AKT信号通路对骨肉瘤细胞黏附和迁移的作用[J].肿瘤学杂志,2019,25(4):320-324. |
| 溶血磷脂酸通过调控PI3K/AKT信号通路对骨肉瘤细胞黏附和迁移的作用 |
| Lysophosphatidic Acid Promotes Adhesion and Migration of Osteosarcoma Cells through PI3K/AKT Signaling Pathway |
| 投稿时间：2017-06-12 |
| DOI：10.11735/j.issn.1671-170X.2019.04.B007 |
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| 中文关键词: 溶血磷脂酸 骨肉瘤 黏附 迁移 PI3K/AKT信号通路 |
| 英文关键词:LPA osteosarcoma adhesion migration PI3K/AKT pathway |
| 基金项目:广东省省级科技计划项目（2014A020212378） |
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| 中文摘要: |
| 摘 要：［目的］ 研究溶血磷脂酸（lysophosphatidic acid，LPA）在骨肉瘤细胞黏附和迁移中的作用及其相关分子机制。［方法］通过黏附和划痕实验观察细胞黏附和迁移能力；q-PCR和Western blot检测骨肉瘤细胞系(Mg-63，SaOS-2，HOS-8603)中溶血磷脂酸受体（lysophosphatidic acid receptor，LPAR）的表达，分析LPAR的表达与肿瘤细胞迁移侵袭之间的关系；黏附和划痕实验检测LPA处理后对骨肉瘤细胞黏附和迁移的影响；Western Blot检测LPA对骨肉瘤细胞黏附和迁移相关信号通路蛋白的影响。［结果］ 细胞黏附和迁移能力检测结果显示Mg-63细胞的黏附和迁移能力最强，SaOS-2细胞黏附和迁移能力最弱。细胞中LPAR检测结果显示Mg-63、SaOS-2、HOS-8603三株细胞中LPAR1、LPAR2、LPAR3均有不同程度的表达，其中Mg-63细胞中LPAR1、LPAR2、LPAR3表达均为最高。LPA处理SaOS-2细胞后，其黏附和迁移能力均明显下降；Western Blot结果显示LPA处理后，SaOS-2细胞中PI3K、p-AKT、CD44表达均明显上调。［结论］ LPA可能主要通过PI3K/AKT通路促进骨肉瘤细胞黏附和迁移。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of lysophosphatidic acid(LPA) on the adhesion and migration of osteosarcoma cells and the related molecular mechanisms. ［Methods］ Osteosarcoma Mg-63，SaOS-2 and HOS-8603 cells were treated with LPA and the cell adhesion and migration were detected by adhesion and scratches assay. The expression of lysophospholitidic acid receptor (LPAR) mRNA and protein in osteosarcoma cells was detected by q-PCR and Western blot，respectively. The relationship between the expression of LPAR and cell migration was analyzed. The expression of proteins related to cell adhesion and migration was detected by Western blot. ［Results］ The adhesion and migration of Mg-63 cells were more marked than that of other two cell lines. The expression of LPAR1，LPAR2 and LPAR3 was all detected in Mg-63，SaOS-2 and HOS-8603 cells，and the expression in Mg-63 cells was the highest. After LPA treatment，the adhesion and migration of SaOS-2 cells decreased significantly. Western blot showed that the expression of PI3K，p-AKT，CD44 was significantly up-regulated after LPA treatment. ［Conclusion］ LPA promotes adhesion and migration of osteosarcoma cells，in which the PI3K/AKT signaling pathway is involved. |
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