| 钱红燕,杨俐萍,赵文静.Angiomotin like-2 mRNA在胃癌组织中的定量分析及其临床意义[J].肿瘤学杂志,2018,24(2):81-85. |
| Angiomotin like-2 mRNA在胃癌组织中的定量分析及其临床意义 |
| Angiomotin Like-2 mRNA Level in Gastric Cancer Tissues and Its Clinical Significance |
| 投稿时间：2017-06-19 |
| DOI：10.11735/j.issn.1671-170X.2018.02.B001 |
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| 中文关键词: 类血管动蛋白-2 胃肿瘤 实时荧光定量PCR |
| 英文关键词:angiomotin like-2(AMOTL2) gastric cancer real-time fluorescence quantitative PCR |
| 基金项目:南通市科技局前沿与关键技术创新项目(MS22015005)；南通市卫计委青年基金(WQ2015056)；南通市“科教兴卫”工程临床医学中心（创新平台）(通卫科教［2012］9号)；南通市指导性科技计划（HS149071）；南通市科教强卫工程项目（通卫科教［2017］35号） |
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| 中文摘要: |
| 摘 要：［目的］ 定量分析类血管动蛋白-2（angiomotin like-2，AMOTL2）mRNA表达水平的改变与胃癌发生发展的关系及其临床意义。［方法］ 免疫组化染色检测5对胃癌石蜡切片中AMOTL2蛋白的表达；实时荧光定量PCR(qPCR)检测30对胃癌与癌旁组织及25例胃癌患者血浆中AMOTL2的表达；回顾性分析临床病理特征及随访资料，并结合AMOTL2 mRNA表达水平，用GraphPad Prism 6.0软件绘制胃癌患者的5年生存曲线。［结果］ 免疫组化染色显示AMOTL2蛋白在胃癌及其癌旁组织均有表达，但是癌区的表达明显低于癌旁。qPCR定量检测结果显示AMOTL2 mRNA在胃癌组织中明显低于癌旁组织(P=0.043)；在胃癌患者血浆中的AMOTL2 mRNA水平亦明显低于健康组(P=0.010)。胃癌组织中AMOTL2 mRNA水平与肿瘤直径呈负相关 (r=-0.569，P=0.001)；生存随访分析显示至术后60个月，AMOTL2 mRNA低水平组患者术后总生存率明显低于高水平组(P=0.038)。［结论］ AMOTL2在胃癌组织及血浆中均呈低表达，其mRNA表达水平与肿瘤直径呈负相关，且胃癌组织中AMOTL2低表达组生存率明显低于高表达组，提示AMOTL2可能是一个新的胃癌潜在预后标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To quantitatively analyze angiomotin like-2(AMOTL2) mRNA level that is potentially involved in gastric cancer development and to investigate its clinical significance.［Methods］ The expression of AMOTL2 protein in paraffin-embedded tumor tissues from five patients having gastric cancer was detected by immunohistochemical(IHC) staining. AMOTL2 mRNA level from 30 pairs of tumor and peritumoral tissue samples and plasma samples of 25 patients having gastric cancer was tested by real-time fluorescence quantitative PCR(qPCR). The relationship between AMOTL2 mRNA level and clinical pathologic phenotype was analyzed，and survival was analyzed using GraphPad Prism 6.0 software.［Results］ IHC analysis showed that AMOTL2 protein was expressed in both tumor and peritumoral tissues，but that the expression level was lower in the tumor tissues than in the peritumoral tissues. In addition，the mRNA level of AMOTL2 was significantly lower in the tumor tissues(P=0.043) than in peritumoral tissues. Similarly，AMOTL2 mRNA level in the plasma of patients having gastric cancer was significantly lower than that in healthy controls(P=0.010). The reduction of AMOTL2 mRNA level was negatively correlated with tumor size increases(r=-0.569，P=0.001). Furthermore，survival analysis showed that up to sixty months after surgery，the survival rate in the group of high level of AMOTL2 mRNA was significantly lower than that of low level of AMOTL2 mRNA(P=0.038).［Conclusion］ AMOTL2 mRNA level is lower in gastric cancer tissues and plasma. And the gene expression pattern of AMOTL2 is associated with the tumor size negatively. Furthermore，the low level of AMOTL2 mRNA in the gastric cancer tissue is disadvantageous to the survival of patients after surgery. AMOTL2 might be a potential biomarker for the prognosis of gastric cancer. |
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