| 赵 洁,刘晓颖.Her-2阳性与Her-2阴性人乳腺癌组织蛋白质组的差异表达[J].肿瘤学杂志,2017,23(9):794-797. |
| Her-2阳性与Her-2阴性人乳腺癌组织蛋白质组的差异表达 |
| Different Expression of Proteome in Her-2-Positive and Her-2-Negative Human Breast Cancer |
| 投稿时间：2016-12-30 |
| DOI：10.11735/j.issn.1671-170X.2017.09.B011 |
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| 中文关键词: 乳腺癌 蛋白质组 差异表达 Her-2 |
| 英文关键词:breast cancer proteomics differential expression Her-2 |
| 基金项目:湖南省科技厅基金资助项目（2014FJ3036） |
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| 中文摘要: |
| 摘 要：［目的］ 通过蛋白质组学方法探讨Her-2阳性与Her-2阴性人乳腺癌组织蛋白质组的差异表达。［方法］ 采用顺序抽提的方法将细胞总蛋白分级为水溶性和疏水性两个组分，并应用双向电泳方法对其进行分离，以获取蛋白质的表达谱；通过软件分析，找出表达量存在显著差异的蛋白质点；再应用基质辅助激光解吸电离飞行时间质谱与生物信息学相结合的方法对差异表达蛋白点进行分析鉴定。［结果］ 双向电泳图谱分析表明对水溶性蛋白质和疏水性蛋白都取得了很好的分离，并成功鉴定出7个差异表达蛋白，其中4个下调，3个上调。［结论］ 乳腺癌的发生发展与蛋白的表达改变有关，这些蛋白质参与信号传导、浸润转移及能量代谢等多个方面。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the different expression of proteome in Her-2-positive and Her-2-negative human breast cancer by proteomic technologies. ［Methods］ Sequential protein extraction was used and proteins were fractionated as soluble or hydrop hobic fractions which were analyzed by 22DE. Spots were detected and evaluated by 2D platinum Software 5. 0 and the spots with different expression levels were subjected to MS analysis. ［Results］ 22DE patterns demonstrated a good resolution for soluble proteins and for hydrop hobic proteins. Moreover，7 significant proteins were identified，among which 4 were down regulated and 3 were up regulated. ［Conclusion］ The occurrence and development of breast cancer may be related to the expression change of proteins，which are involved in signal transduction，invasion and metastasis，as well as energy metabolism. |
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