| 季文豪,杨诗舟,毛伟敏.食管鳞状细胞癌组织中miR-34a的表达及其临床意义[J].肿瘤学杂志,2016,22(12):1026-1031. |
| 食管鳞状细胞癌组织中miR-34a的表达及其临床意义 |
| The Expression of MiR-34a in Esophageal Squamous Cell Carcinoma and Its Clinical Significance |
| 投稿时间：2015-11-22 |
| DOI：10.11735/j.issn.1671-170X.2016.12.B009 |
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| 中文关键词: 食管肿瘤 鳞状细胞癌 microRNA-34a 无进展生存期 总生存期 |
| 英文关键词:esophageal neoplasms squamous cell carcinoma microRNA-34a progression-free survival(PFS) overall survival(OS) |
| 基金项目:国家自然科学基金资助项目（81172081，81572822）；浙江省科技重大专项（2011C13039-1）；国家卫生计生委科学研究基金—浙江省医药卫生重大科技计划项目（WKJ-ZJ- 1505）；浙江省胸部肿瘤（肺、食管）诊治技术研究重点实验室专项基金项目；浙江省卫生高层次创新人才培养工程项目；浙江省新世纪151人才工程重点资助项目 |
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| 中文摘要: |
| 摘 要：［目的］ 研究食管鳞状细胞癌组织中miR-34a表达水平及其临床意义。［方法］ 采用实时反转录聚合酶链反应(reverse transcription-polymerase chain reaction，RT-PCR)技术检测75例食管鳞状细胞癌组织及配对癌旁组织中miR-34a的表达水平，2-ΔΔCt法计算食管鳞状细胞癌组织中miR-34a相对表达量，进一步结合临床、病理及随访资料进行统计分析。［结果］ miR-34a在75例食管鳞癌组织中相对表达量高于癌旁组织；多因素分析显示，吸烟史和临床分期是食管鳞癌组织中miR-34a表达的独立影响因素。miR-34a低表达组的无进展生存期和总生存期均明显劣于高表达组；多因素分析显示miR-34a低表达是无进展生存期和总生存期缩短的独立影响因素。［结论］ 食管鳞癌组织中miR-34a表达水平可作为预测预后的一项重要指标。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of miR-34a in esophageal squamous cell carcinoma(ESCC) and its clinical significance. ［Methods］ The expression of miR-34a in 75 cases with ESCC and adjacent cancer tissues was tested by reverse transcription-polymerase chain reaction(RT-PCR). 2-ΔΔCt method was used to calculate the relative quantitative expression of miR-34a，which was analyzed together with clinicopathological and follow-up data. ［Results］ The expression of miR-34a was higher in the tumor tissues than that in cancer adjacent tissues. Multivariate analysis showed that smoking and clinical stage were independent factors influencing the expression of miR-34a in ESCC.Progression-free survival and overall survival in lower miR-34a expression group were worse than those in higher miR-34a expression group. Multivariate analysis indicated that lower miR-34a expression was independently associated with poorer progression-free survival and overall survival. ［Conclusions］ MiR-34a might be an important indicator for prediction of prognosis of ESCC. |
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