| 陈洪亮,陈志良,应晓江.微小RNA-143在结直肠癌组织中的表达及其对结肠癌细胞凋亡的影响[J].肿瘤学杂志,2015,21(9):748-752. |
| 微小RNA-143在结直肠癌组织中的表达及其对结肠癌细胞凋亡的影响 |
| Expression of MiR-143 in Colorectal Cancer and Its Role in Cell Apoptosis of Colonic Cancer Cell Lines |
| 投稿时间：2014-12-10 |
| DOI：10.11735/j.issn.1671-170X.2015.09.B010 |
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| 中文关键词: 结直肠肿瘤 miR-143 细胞凋亡 |
| 英文关键词:colorectal neoplasms miR-143 cell apoptosis |
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| 中文摘要: |
| 摘 要：［目的］ 探讨微小RNA-143（miR-143）在结直肠癌组织中的表达及临床意义，以及上调miR-143对于结直肠癌细胞凋亡的影响。［方法］ 采用实时荧光定量聚合酶链反应（real-time quantitative reverse transcription-PCR，real-time PCR）的方法在45例结直肠癌患者组织标本中检测miR-143的表达水平，结合病理资料分析其临床意义。HCT-116细胞转染miR-143 mimics后，用流式细胞术检测miR-143对凋亡的影响。［结果］ miR-143在结肠癌组织中的相对表达量为0.48（0.24~0.91），在癌旁组织中的表达量为1.64（0.83~1.87），差异有统计学意义（P<0.001），且在黏液癌患者中下调更明显（P<0.05）。流式细胞术检测发现，与对照组的细胞凋亡率（20.00%±2.00%）相比，实验组的细胞凋亡率升高至31.83%±2.02%，差异有统计学意义（P=0.002）。［结论］ miR-143在结直肠癌组织中低表达，并且与组织学类型有关；miR-143表达上调可促进HCT-116细胞凋亡；miR-143具有潜在抑癌作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of miRNA-143(miR-143) in colorectal cancer and its significance，and to study the effect of up-regulation of miR-143 on cell apoptosis of colonic cancer cell lines.［Methods］ Real-time quantitative reverse transcription-PCR assay was applied to detect the expression level of miR-143 in 45 samples of colorectal cancer tissues. Correlation between miRNA expression levels and clinical characteristics of the patients were also analyzed. HCT-116 cells were transfected with miR-143 mimics，and the cell apoptosis levels were detected by flow cytometry. ［Results］ The relative expression level of miR-143 in colorectal cancer tissues was 0.48(0.24～0.91)，which was significantly different compared with that in cancer adjacent tissues［1.64(0.83～1.87)，P<0.001］. The expression level of miR-143 was significantly lower in mucinous carcinoma compared with that in adenocarcinoma (P<0.05). The result of flow cytometry assay suggested that the ratio of apoptotic cells was(31.83%±2.02%) in HCT-116 cells with overexpression of miR-143(experimental group)，which was significantly higher compared with that in control group［(20.00%±2.00%)，P=0.002］. ［Conclusion］ The expression level of miR-143 is low in colorectal cancer tissue，and is related to the histologic type. Moreover，up-regulation of miR-143 might promote the cell apoptosis of HCT-116 cells，which suggest miR-143 has a potential role on inhibition of tumor. |
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