| 赵 丽,李泳群,冯华松.氩氦冷冻消融治疗前后荷瘤鼠程序性死亡配体-1的表达变化[J].肿瘤学杂志,2015,21(7):567-571. |
| 氩氦冷冻消融治疗前后荷瘤鼠程序性死亡配体-1的表达变化 |
| Changes of Expression of Programmed Death Ligand-1(PD-L1) in Tumor-bearing Mice Before and After Argon-Helium Cryoablation |
| 投稿时间：2014-05-22 |
| DOI：10.11735/j.issn.1671-170X.2015.07.B008 |
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| 中文关键词: 冷冻治疗 肺肿瘤 程序性死亡配体-1 |
| 英文关键词:cryoablation lung neoplasms programmed death ligand-1(PD-L1) |
| 基金项目:首都医学发展科研基金(NO.2007-2025) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨冷冻治疗对荷瘤鼠单个核细胞表面程序性死亡配体-1（PD-L1）分子表达的影响。［方法］ 流式细胞术检测冷冻术后2h、3d、7d对照组和冷冻治疗组荷瘤鼠外周血和脾脏单个核细胞表面PD-L1分子的动态表达。［结果］ 对照组单个核细胞表面PD-L1表达水平逐渐升高（P<0.01），冷冻治疗组逐渐下降（P<0.05）；与对照组相比，冷冻治疗术后2h PD-L1分子表达有一过性升高，但3d、7d后显著下降(P<0.01)。［结论］ 单个核细胞表面PD-L1分子表达水平随Lewis肺癌进展逐渐增强，冷冻治疗可能可以通过降低PD-L1分子的表达水平，提高抗肿瘤免疫活性。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of cryoablation on the expression of programmed death ligand-1(PD-L1) on the surface of mononuclear cells of tumor-bearing mice. ［Methods］ The expression of PD-L1 on the mononuclear cells of peripheral blood and spleen in control group and cryotherapy group was determined by flow cytometry after 2 hours，3 days，7 days of Argon-Helium cryoablation. ［Results］ The expression level of PD-L1 on the surface of mononuclear cells gradually increased in the control group(P<0.01)，but it gradually decreased in the cryotherapy group(P<0.05).Compared with control group，the expression level of PD-L1 increased after 2 hours of cryoablation，but significantly decreased after 3 days and 7 days(P<0.05). ［Conclusion］ The expression level of PD-L1 on the surface of mononuclear cells increases gradually with tumor progression of Lewis lung cancer，and Argon-Helium cryoablation possibly enhances the anti-tumor immune activity by reducing the expression level of PD-L1. |
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