赵 丽,李泳群,冯华松.氩氦冷冻消融治疗前后荷瘤鼠程序性死亡配体-1的表达变化[J].肿瘤学杂志,2015,21(7):567-571.
氩氦冷冻消融治疗前后荷瘤鼠程序性死亡配体-1的表达变化
Changes of Expression of Programmed Death Ligand-1(PD-L1) in Tumor-bearing Mice Before and After Argon-Helium Cryoablation
投稿时间:2014-05-22  
DOI:10.11735/j.issn.1671-170X.2015.07.B008
中文关键词:  冷冻治疗  肺肿瘤  程序性死亡配体-1
英文关键词:cryoablation  lung neoplasms  programmed death ligand-1(PD-L1)
基金项目:首都医学发展科研基金(NO.2007-2025)
作者单位
赵 丽 安徽医科大学海军临床学院 
李泳群 海军总医院 
冯华松 海军总医院 
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中文摘要:
      摘 要:[目的] 探讨冷冻治疗对荷瘤鼠单个核细胞表面程序性死亡配体-1(PD-L1)分子表达的影响。[方法] 流式细胞术检测冷冻术后2h、3d、7d对照组和冷冻治疗组荷瘤鼠外周血和脾脏单个核细胞表面PD-L1分子的动态表达。[结果] 对照组单个核细胞表面PD-L1表达水平逐渐升高(P<0.01),冷冻治疗组逐渐下降(P<0.05);与对照组相比,冷冻治疗术后2h PD-L1分子表达有一过性升高,但3d、7d后显著下降(P<0.01)。[结论] 单个核细胞表面PD-L1分子表达水平随Lewis肺癌进展逐渐增强,冷冻治疗可能可以通过降低PD-L1分子的表达水平,提高抗肿瘤免疫活性。
英文摘要:
      Abstract:[Purpose] To investigate the effect of cryoablation on the expression of programmed death ligand-1(PD-L1) on the surface of mononuclear cells of tumor-bearing mice. [Methods] The expression of PD-L1 on the mononuclear cells of peripheral blood and spleen in control group and cryotherapy group was determined by flow cytometry after 2 hours,3 days,7 days of Argon-Helium cryoablation. [Results] The expression level of PD-L1 on the surface of mononuclear cells gradually increased in the control group(P<0.01),but it gradually decreased in the cryotherapy group(P<0.05).Compared with control group,the expression level of PD-L1 increased after 2 hours of cryoablation,but significantly decreased after 3 days and 7 days(P<0.05). [Conclusion] The expression level of PD-L1 on the surface of mononuclear cells increases gradually with tumor progression of Lewis lung cancer,and Argon-Helium cryoablation possibly enhances the anti-tumor immune activity by reducing the expression level of PD-L1.
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