| 李家树,李春华,吴兴萍.支气管肺泡灌洗液DNA异倍体、p53基因检测在肺癌诊断中的应用研究[J].肿瘤学杂志,2015,21(7):559-562. |
| 支气管肺泡灌洗液DNA异倍体、p53基因检测在肺癌诊断中的应用研究 |
| Detection of DNA Heteroploid and p53 Gene Mutation of the bronchoalveolar Lavage (BALF) in the Diagnosis of Lung Cancer |
| 投稿时间：2014-08-16 |
| DOI：10.11735/j.issn.1671-170X.2015.07.B006 |
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| 中文关键词: 支气管肺泡灌洗液 DNA p53 流式细胞术 肺肿瘤 |
| 英文关键词:bronchoalveolar lavage fluid DNA p53 flow cytometry lung neoplasms |
| 基金项目:江苏省连云港市卫生局课题（09005） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨支气管肺泡灌洗夜（bronchoalveolar lavage fluid，BALF）中DNA异倍体及p53基因突变在肺癌诊断中的应用。［方法］ 86例患者分为周围型肺癌组和肺良性疾病组，均分别行纤维支气管镜检查并收集肺泡灌洗液，流式细胞仪检测肺泡灌洗液中DNA异倍体、p53基因突变情况。［结果］ p53基因突变、DNA异倍体阳性及p53基因突变并DNA异倍体阳性检出率在肺癌组明显高于肺良性疾病组（P＜0.05）。p53基因突变阳性敏感性较高、特异性较低，DNA异倍体阳性敏感性及特异性均较高，而p53基因突变并DNA异倍体阳性虽然敏感性较低（27.50%），但是特异性高（91.30%）。［结论］ 肺泡灌洗液p53基因突变并DNA异倍体阳性具有较高特异性，能够进一步指导临床医生诊疗决策。 |
| 英文摘要: |
| Abstract：［Purpose］ ?壮o investigate the diagnostic value of DNA heteroploid and p53 gene mutation of the bronchoalveolar lavage (BALF) in lung cancer patients. ［Methods］ Eighty-six patients were divided into peripheral type lung cancer group and lung benign disease group，and received fiber bronchoscope examination，and BALF samples were obtained and analyzed for DNA heteroploid and p53 gene mutation by flow cytometry. ［Results］ ?妆53 gene mutation，positive of DNA heteroploid，and combination of p53 gene mutation and positive of DNA heteroploid，were higher in lung cancer group than those in lung benign disease group（P＜0.05）. ?妆53 gene mutation had high sensitivity and low specificity，positive of DNA heteroploid had both high sensitivity and high specificity，while combination of p53 gene mutation and DNA heteroploid had low sensitivity (27.50%)，but high specificity(91.30%). ［Conclusion］ Combination of p53 gene mutation and positive of DNA heteroploid has high specificity in BALF，which might guide the further diagnosis and treatment decision of clinicians. |
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