| 温鹏路,马 亮,葛光炬.人β防御素-1联合冬凌草甲素对膀胱癌T24 细胞生长抑制作用的研究[J].肿瘤学杂志,2014,20(12):1006-1011. |
| 人β防御素-1联合冬凌草甲素对膀胱癌T24 细胞生长抑制作用的研究 |
| Inhibition Effect of Human Beta-defensin-1 Combined with Oridonin on Bladder Cancer T24 Cell |
| 投稿时间：2014-08-19 |
| DOI：10.11735/j.issn.1671-170X.2014.12.B008 |
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| 中文关键词: β防御素-1 冬凌草甲素 膀胱肿瘤 细胞毒性 |
| 英文关键词:human beta-defensin-1 oridonin bladder neoplasms cytotoxicity |
| 基金项目:浙江省中医药科技计划项目（2007CA064） |
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| 中文摘要: |
| 摘 要：［目的］探讨人β防御素-1联合冬凌草甲素对膀胱癌T24细胞的生长抑制作用及机制。［方法］以不同浓度的β防御素-1、冬凌草甲素单独或联合作用于体外培养的T24细胞，MTT法检测细胞生长抑制率，应用Hoechst 33258 荧光染色法以流式细胞仪观察细胞凋亡，显微镜下观察细胞的形态学变化。［结果］人β防御素-1及冬凌草甲素均可抑制T24 细胞的生长，引起T24细胞溶解坏死，并呈现明显的浓度—效应与时间—效应关系。［结论］人β防御素-1、冬凌草甲素均对膀胱癌具有明显的体外抗肿瘤作用，两者联合能对膀胱癌细胞起协同杀伤作用，杀伤机制以细胞毒性作用为主。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the inhibitory effect of human beta-defensin-1 combined with oridonin on bladder cancer T24 cells and its mechanism. ［Methods］ Different concentrations of human beta-defensin-1 and oridonin were added in culture medium. The inhibitory rate of T24 cells was measured by MTT assay，cell apoptosis was observed by flowcytometry(FCM) and Hoechst 33258 fluorescence staining. Cell morphology was observed by microscopy. ［Results］ Human beta-defensin-1 combined with oridonin inhibited the growth of T24 cells and induced cell cytolysis significantly. The suppression was both in a time- and dose-dependent manner. ［Conclusion］ Human beta-defensin-1 combined with oridonin has inhibitory effect on bladder cancer T24 cell in vitro，which might be associated with the cell cytotoxicity. |
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