| 江 皓,赵鹏军,冯建国.重楼皂苷Ⅰ对胰腺癌PANC-1细胞体外放射敏感性的影响[J].肿瘤学杂志,2014,20(6):483-487. |
| 重楼皂苷Ⅰ对胰腺癌PANC-1细胞体外放射敏感性的影响 |
| Radiosensitivity of Paris Saponin I on Pancreatic Carcinoma Cell Line PANC-1 in Vitro |
| 投稿时间：2013-12-21 |
| DOI：10.11735/j.issn.1671-170X.2014.06.B010 |
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| 中文关键词: 重楼皂苷Ⅰ 胰腺肿瘤 放射敏感性 |
| 英文关键词:paris saponin Ⅰ pancreatic neoplasms radiosensitivity |
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| 中文摘要: |
| 摘 要：［目的］ 探讨重楼皂苷Ⅰ(PSⅠ)对胰腺癌PANC-1细胞的放射增敏作用及可能机制。［方法］ MTT法检测PSⅠ对PANC-1细胞的增殖抑制作用。克隆形成实验检测PSⅠ对PANC-1细胞的放射增敏效应。流式细胞仪检测细胞周期变化。Annexin Ⅴ-PI双染法检测细胞凋亡。Western Blot法检测caspase-3、Bax、Bcl-2蛋白表达改变。［结果］ 不同浓度PSⅠ对PANC-1细胞有不同程度的体外抑制作用。1μg/ml PSⅠ联合放射线明显降低PANC-1细胞克隆形成率。增敏组凋亡率及G2/M期细胞比例较对照组明显增多(P＜0.01)。PSⅠ作用后细胞caspase-3、Bax蛋白表达增加，Bcl-2蛋白表达降低。［结论］ PSⅠ对胰腺癌PANC-1细胞具有放射增敏作用，其机制可能与诱导caspase-3、Bax蛋白表达增加，Bcl-2蛋白表达降低，引起细胞G2/M 期阻滞，促进细胞凋亡有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the radiosensitization of paris saponinⅠ( PSⅠ) on pancreatic carcinoma cell line PANC-1 and its possible mechanism.［Methods］ MTT assay was used to measure the effect of PSⅠ on PANC-1 cell proliferation. Clonogenic assay was performed to determine the radiosensitizing effect of PSⅠ on PANC-1 cell line. The cell cycle was measured by flow cytometry and apoptosis was measured by Annexin Ⅴ-PI. Caspase-3，Bax and Bcl-2 protein expression were detected by Western Blot. ［Results］PSⅠ inhibited PANC-1 cell line proliferation. Radiation with PSⅠ(1μg/ml) decreased cloning efficiency significantly. The radiosensitization group had a higher apoptosis rate and G2/M phase cells than control group(P＜0.01) . Western Blot showed caspase-3 and Bax protein expression increased after the treatment of PSⅠ， but Bcl-2 protein expression decreased. ［Conclusion］ PSⅠ could enhance the radiosentivity of pancreatic carcinoma cell line PANC-1，which might induce caspase-3，Bax proteins increasing，Bcl-2 protein expression decreasing，and cause G2/M phase arrest and promote cell apoptosis. |
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