| 白瑞霞,赵鹏伟,韩晓芳.采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1 期细胞的hnRNPA2/B1蛋白[J].肿瘤学杂志,2013,19(7):505-508. |
| 采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1 期细胞的hnRNPA2/B1蛋白 |
| Identification of hnRNPA2/B1 Protein in G1 Phase from HepG2 Using Proteomics Technique |
| 投稿时间：2013-01-07 |
| DOI：10.11735/j.issn.1671-170X.2013.07.B001 |
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| 中文关键词: 肝肿瘤 HepG2 蛋白质组学 hnRNPA2/B1蛋白 |
| 英文关键词:liver neoplasms HepG2 proteomics hnRNPA2/B1 protein |
| 基金项目: |
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| 摘要点击次数: 2483 |
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| 中文摘要: |
| 摘 要：［目的］ 采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1期细胞内的不均一性核糖核蛋白A2/B1（hnRNPA2/B1）蛋白。［方法］ 提取肝癌细胞株HepG2的G1期细胞的蛋白质，采用双向电泳方法分离蛋白质，应用图像扫描仪及质谱(MALDI-TOF-MS)分析鉴定HepG2的G1期细胞表达的蛋白质。［结果］ 采用双向电泳技术分离，并用质谱成功鉴定出HepG2的G1期细胞的hnRNPA2/B1。［结论］ 肝癌细胞株HepG2的G1期细胞内的hnRNPA2/B1蛋白的成功鉴定，为探讨其在肝癌细胞生长和增殖中的作用奠定了基础，并为临床上肿瘤的治疗提供参考依据。 |
| 英文摘要: |
| Abstract: ［Purpose］ To identify hnRNPA2/B1 protein in G1 phase from HepG2 by the proteomics methods. ［Methods］ G1 phase Proteins of HepG2 cells were extracted and separated by two-dimensional electrophoresis，and the hnRNPA2/B1 proteins were identified by image scanner and MALDI-TOF-MS. ［Results］ HnRNPA2/B1 protein in G1 phase from HepG2 was successfully separated and identified. ［Conclusion］ The hnRNPA2/B1 protein in G1 phase from HepG2 is identified successfully. It can provide the basis of the treatment for tumor. |
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