白瑞霞,赵鹏伟,韩晓芳.采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1 期细胞的hnRNPA2/B1蛋白[J].肿瘤学杂志,2013,19(7):505-508. |
采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1 期细胞的hnRNPA2/B1蛋白 |
Identification of hnRNPA2/B1 Protein in G1 Phase from HepG2 Using Proteomics Technique |
投稿时间:2013-01-07 |
DOI:10.11735/j.issn.1671-170X.2013.07.B001 |
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中文关键词: 肝肿瘤 HepG2 蛋白质组学 hnRNPA2/B1蛋白 |
英文关键词:liver neoplasms HepG2 proteomics hnRNPA2/B1 protein |
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中文摘要: |
摘 要:[目的] 采用蛋白质组学技术鉴定肝癌细胞株HepG2的G1期细胞内的不均一性核糖核蛋白A2/B1(hnRNPA2/B1)蛋白。[方法] 提取肝癌细胞株HepG2的G1期细胞的蛋白质,采用双向电泳方法分离蛋白质,应用图像扫描仪及质谱(MALDI-TOF-MS)分析鉴定HepG2的G1期细胞表达的蛋白质。[结果] 采用双向电泳技术分离,并用质谱成功鉴定出HepG2的G1期细胞的hnRNPA2/B1。[结论] 肝癌细胞株HepG2的G1期细胞内的hnRNPA2/B1蛋白的成功鉴定,为探讨其在肝癌细胞生长和增殖中的作用奠定了基础,并为临床上肿瘤的治疗提供参考依据。 |
英文摘要: |
Abstract: [Purpose] To identify hnRNPA2/B1 protein in G1 phase from HepG2 by the proteomics methods. [Methods] G1 phase Proteins of HepG2 cells were extracted and separated by two-dimensional electrophoresis,and the hnRNPA2/B1 proteins were identified by image scanner and MALDI-TOF-MS. [Results] HnRNPA2/B1 protein in G1 phase from HepG2 was successfully separated and identified. [Conclusion] The hnRNPA2/B1 protein in G1 phase from HepG2 is identified successfully. It can provide the basis of the treatment for tumor. |
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