卿 菁,赵素萍,蒋卫红.融合自杀基因对鼻咽癌细胞的体内杀伤作用[J].肿瘤学杂志,2013,19(6):448-453.
融合自杀基因对鼻咽癌细胞的体内杀伤作用
Killing Effect of Fusion Suicide Genes on Nasopharyngeal Carcinoma Cells in vivo
投稿时间:2013-04-24  
DOI:10.11735/j.issn.1671-170X.2013.06.B009
中文关键词:  鼻咽肿瘤  动物模型  自杀基因
英文关键词:nasopharyngeal neoplasms  animal model  suicide gene
基金项目:
作者单位
卿 菁 中南大学湘雅医院\宁波市第一医院 
赵素萍 中南大学湘雅医院 
蒋卫红 中南大学湘雅医院 
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中文摘要:
      摘 要:[目的] 研究融合自杀基因对鼻咽癌细胞的体内杀伤作用。[方法] 培养鼻咽癌CNE-2细胞,建立裸鼠荷瘤模型。随机分为6组,每组5只,A组:脂质体包裹质粒瘤内注射瘤内组;B组:脂质体包裹质粒瘤内注射+肿瘤局部照射γ射线(总剂量9Gy)组;C组:脂质体包裹质粒瘤内注射+腹腔注射5氟胞嘧啶(5-Fc)组;D组:脂质体包裹质粒瘤内注射+肿瘤局部照射γ射线(总剂量9Gy)+腹腔注射5-Fc组;E组:肿瘤局部照射γ射线(总剂量9Gy)+腹腔注射5-Fc,以及空白对照组。记录肿瘤体积变化;计算各组肿瘤抑制率,绘制肿瘤生长曲线;取肿瘤组织进行病理观察;鉴定肿瘤组织中CD/UPRT.UL49基因。肿瘤体积变化的比较采用单向方差分析,组间多重比较采用LSD法。[结果] 成功建立鼻咽癌裸鼠荷瘤模型,生长曲线显示:5组瘤体积差异有统计学意义(F=720.684,P<0.01),测序结果证实,除E组外,其余各处理组均检测到目的基因,且Western blot检测结果示:B和D组肿瘤组织内自杀基因的表达高于其余各组。病理切片证实肿块为低分化鳞癌,其中D组肿瘤组织在干预作用下出现明显坏死。[结论] E6.BARF1p.CD/UPRT.UL49自杀基因载体联合前药5-Fc在放射线的作用下,可明显抑制鼻咽癌移植瘤的生长,转染该融合自杀基因可增强放疗敏感性,为鼻咽癌的基因—放射治疗开辟了新思路。
英文摘要:
      Abstract:[Purpose] To explore the killing effect of fusion suicide genes in human nasopharyngeal carcinoma(NPC) in vivo. [Methods] NPC CNE-2 cell line was hosted to establish mice model with tumor which were randomly divided into 6 groups,where there were 5 mice with different interventions. The volumes of tumor were measured and compared,the tumor growth inhibition rates were calculated. The tumor tissue was taken out for analysis by Hematoxylin-Eosin staining,and the existence of CD/UPRT.UL49 was detected in tumor tissue. Analysis of variance was used for the comparison of tumor volume,and the LSD method was used for the multiple comparison among groups. [Results] Tumor bearing mice models with NPC were successfully established. The tumor sizes of five groups showed significantly difference(F=720.684,P<0.01). The sequencing testing proved the existence of suicide gene in all the treatment groups except group E,and Western blot showed the expression of suicide genes in group B and D was higher than that in other groups. The pathologic section showed low differentiated squamous cell carcinoma in group B,and tumor tissues showed obvious necrosis in group D. [Conclusion] Under the radiation,suicide genes of E6.BARF1p.CD/UPRT.UL49 combined with 5-Fc obviously suppressed the growth of transplant tumor,and it was proved fusion suicide genes play a role in increasing sensitivity of tumor cells to radiation,which set up new ideas for the gene-radiation therapy for NPC.
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