王梅梅,熊亚南,朱丽华.EB病毒阳性肺癌组织中LMP-1、p53、Bcl-2及MMP-9表达[J].肿瘤学杂志,2013,19(6):431-434.
EB病毒阳性肺癌组织中LMP-1、p53、Bcl-2及MMP-9表达
Expressions of LMP-1,p53,Bcl-2 and MMP-9 in Tissues of Lung Cancer with Epstein-Barr Virus Infection
投稿时间:2012-09-20  
DOI:10.11735/j.issn.1671-170X.2013.06.B005
中文关键词:  肺肿瘤  EB病毒小RNA(EBER-1)  原位杂交  潜伏感染膜蛋白-1(LMP-1)  p53  Bcl-2  基质金属蛋白酶-9(MMP-9)
英文关键词:lung neoplasms  EBV encoded small RNA-1(EBER-1)  in situ hybridization  latent infection membrane protein 1(LMP-1)  p53  Bcl-2  matrix matalloproteinases-9(MMP-9)
基金项目:河北省自然科学基金资助项目(H2013209180);唐山市科技计划项目(121302136)。
作者单位
王梅梅 河北联合大学基础医学院 
熊亚南 河北联合大学基础医学院 
朱丽华 河北联合大学基础医学院 
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中文摘要:
      摘 要:[目的] 检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV encoded small RNA-1,EBER-1)。免疫组织化学的方法检测 EBER-1阳性和阴性肺癌组织中LMP-1、 p53、Bcl-2及MMP-9的表达。[结果] 108例肺癌组织中EBER-1阳性36例,阳性率33.3%;22例癌旁正常肺组织中EBER-1阳性1例,阳性率4.5%,差异有统计学意义(P<0.01)。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1%和4.2%,差异无统计学意义(P>0.05);在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积(AA)和积分光密度(IOD)均高于阴性组,差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9 AA和IOD均高于阴性组,但差异无统计学意义(P>0.05)。[结论] EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达,进而在肺癌的发生、发展和转移中发挥作用。
英文摘要:
      Abstract: [Purpose] To investigate the expressions of Epstein-Barr virus (EBV) latent infection membrane protein 1(LMP-1),p53,Bcl-2,matrix matalloproteinases-9(MMP-9) in tissues of lung cancer with EBV infection,and to explore their relationship with carcinogenesis of lung cancer. [Methods] EBV encoded small RNA-1 (EBER-1) in 108 tissue samples of lung cancer and 22 cases of cancer adjacent tissues were detected by in situ hybridization. The expressions of LMP-1,p53,Bcl-2 and MMP-9 in EBV positive and negative cases were detected immunohistochemically. [Results] Positive expression of EBER-1 was found in 36 of 108 cases with lung cancer(33.3%), and in 1 of 22 cases of cancer adjacent tissues(4.5%),with significant difference(P<0.01). Positive rate of LMP-1 in EBER-1 positive lung cancer tissues and EBER-1 negative tissues was 11.1% and 4.2% respectively,without significant difference. Average area (AA) and integral optical density(IOD) of p53 and Bcl-2 in EBER-1 positive cases were higher than those in negative tissues,with significant difference. AA and IOD of MMP-9 in EBER-1 positive tissues were higher than those in negative tissues,without significant difference(P>0.05). [Conclusion] EBV might play a role in carcinogenesis and metastasis of lung cancer by influencing the expressions of LMP-1,p53,Bcl-2 and MMP-9.
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