| 王梅梅,熊亚南,朱丽华.EB病毒阳性肺癌组织中LMP-1、p53、Bcl-2及MMP-9表达[J].肿瘤学杂志,2013,19(6):431-434. |
| EB病毒阳性肺癌组织中LMP-1、p53、Bcl-2及MMP-9表达 |
| Expressions of LMP-1，p53，Bcl-2 and MMP-9 in Tissues of Lung Cancer with Epstein-Barr Virus Infection |
| 投稿时间：2012-09-20 |
| DOI：10.11735/j.issn.1671-170X.2013.06.B005 |
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| 中文关键词: 肺肿瘤 EB病毒小RNA(EBER-1) 原位杂交 潜伏感染膜蛋白-1（LMP-1） p53 Bcl-2 基质金属蛋白酶-9（MMP-9） |
| 英文关键词:lung neoplasms EBV encoded small RNA-1(EBER-1) in situ hybridization latent infection membrane protein 1(LMP-1) p53 Bcl-2 matrix matalloproteinases-9(MMP-9) |
| 基金项目:河北省自然科学基金资助项目（H2013209180）；唐山市科技计划项目（121302136）。 |
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| 中文摘要: |
| 摘 要：［目的］ 检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1（LMP-1）、p53、Bcl-2及基质金属蛋白酶-9（MMP-9）表达情况，分析它们与肺癌发生发展的关系。［方法］采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1（EBV encoded small RNA-1，EBER-1）。免疫组织化学的方法检测 EBER-1阳性和阴性肺癌组织中LMP-1、 p53、Bcl-2及MMP-9的表达。［结果］ 108例肺癌组织中EBER-1阳性36例，阳性率33.3％；22例癌旁正常肺组织中EBER-1阳性1例，阳性率4.5％，差异有统计学意义（P<0.01）。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1％和4.2％，差异无统计学意义（P>0.05）；在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积（AA）和积分光密度（IOD）均高于阴性组，差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9 AA和IOD均高于阴性组，但差异无统计学意义（P>0.05）。［结论］ EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达，进而在肺癌的发生、发展和转移中发挥作用。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the expressions of Epstein-Barr virus (EBV) latent infection membrane protein 1(LMP-1)，p53，Bcl-2，matrix matalloproteinases-9(MMP-9) in tissues of lung cancer with EBV infection，and to explore their relationship with carcinogenesis of lung cancer. ［Methods］ EBV encoded small RNA-1 (EBER-1) in 108 tissue samples of lung cancer and 22 cases of cancer adjacent tissues were detected by in situ hybridization. The expressions of LMP-1，p53，Bcl-2 and MMP-9 in EBV positive and negative cases were detected immunohistochemically. ［Results］ Positive expression of EBER-1 was found in 36 of 108 cases with lung cancer(33.3%)， and in 1 of 22 cases of cancer adjacent tissues(4.5%)，with significant difference(P<0.01). Positive rate of LMP-1 in EBER-1 positive lung cancer tissues and EBER-1 negative tissues was 11.1% and 4.2％ respectively，without significant difference. Average area (AA） and integral optical density(IOD) of p53 and Bcl-2 in EBER-1 positive cases were higher than those in negative tissues，with significant difference. AA and IOD of MMP-9 in EBER-1 positive tissues were higher than those in negative tissues，without significant difference(P>0.05). ［Conclusion］ EBV might play a role in carcinogenesis and metastasis of lung cancer by influencing the expressions of LMP-1，p53，Bcl-2 and MMP-9. |
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