| 黄 馨,傅剑华,程 斌.PAR1 mRNA及蛋白在食管鳞状细胞癌中的表达及临床意义[J].肿瘤学杂志,2013,19(5):336-341. |
| PAR1 mRNA及蛋白在食管鳞状细胞癌中的表达及临床意义 |
| Expression of PAR1 in Esophageal Squamous Cell Carcinoma and Its Clinical Significance |
| 投稿时间：2012-09-05 |
| DOI：10.11735/j.issn.1671-170X.2013.05.B2012440 |
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| 中文关键词: 蛋白酶活化受体1 食管肿瘤 鳞状细胞癌 RT-PCR 免疫组织化学 |
| 英文关键词:protease activated receptor (PAR1) esophageal neoplasms squamous cell carcinoma RT-PCR IHC |
| 基金项目:中山大学2010年本科生业余科研Ⅰ项目（1163203） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨PAR1 mRNA及蛋白在食管鳞状细胞癌及其匹配癌旁组织中的表达和临床意义。［方法］ 应用RT-PCR方法检测35例食管鳞状细胞癌患者食管原位癌组织及其匹配癌旁组织PAR1 mRNA的表达水平，并分析其与患者性别、年龄、肿瘤大小、临床分期及是否淋巴结转移等临床指标的关系；应用免疫组织化学法检测55对食管鳞癌组织及其匹配癌旁组织的PAR1蛋白表达情况。［结果］ 食管鳞癌组织PAR1 mRNA的表达水平显著高于其匹配的癌旁组织（146.220±24.790 vs 134.054±23.593，t=-4.17，P<0.01），而且与淋巴结是否转移有关（t=-2.199，P<0.05）；食管鳞癌组织PAR1蛋白表达阳性率显著高于其匹配的癌旁组织（49% vs 3.64%，χ2＝23.04，P<0.001）。［结论］ PAR1 mRNA及蛋白在食管鳞癌中的表达均显著上调，可能参与食管癌的发生发展、侵袭与转移。 |
| 英文摘要: |
| Abstract:［Purpose］ To explore PAR1 mRNA and protein expressions in esophageal squamous cell carcinoma(ESCC) tissues and their matched cancer adjacent tissues. ［Methods］ The expressions of PAR1 mRNA and protein in ESCC tissues and their matched cancer adjacent tissues were detected by RT-PCR and IHC， respectively. Meanwhile， the different expressions of PAR1 mRNA in ESCC tissues between different clinicopathologic features were analyzed.［Results］ The expression level of PAR1 mRNA in ESCC tissues was significantly higher than that in their matched cancer adjacent tissues(146.220±24.790 vs 134.054±23.593，t=-4.17， P<0.01). ESCC patients with lymph node metastasis had a higher expression level of PAR1 mRNA(t=-2.199， P<0.05). Also， there was a higher positive rate of PAR1 protein expression in ESCC tissues(49% vs 3.64%，χ2=23.04，P<0.001). ［Conclusion］ Both PAR1 mRNA and protein expressions are upregu-lated in ESCC tissues， which might closely associate with carcinogenesis and progress of ESCC. |
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