黄 馨,傅剑华,程 斌.PAR1 mRNA及蛋白在食管鳞状细胞癌中的表达及临床意义[J].肿瘤学杂志,2013,19(5):336-341. |
PAR1 mRNA及蛋白在食管鳞状细胞癌中的表达及临床意义 |
Expression of PAR1 in Esophageal Squamous Cell Carcinoma and Its Clinical Significance |
投稿时间:2012-09-05 |
DOI:10.11735/j.issn.1671-170X.2013.05.B2012440 |
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中文关键词: 蛋白酶活化受体1 食管肿瘤 鳞状细胞癌 RT-PCR 免疫组织化学 |
英文关键词:protease activated receptor (PAR1) esophageal neoplasms squamous cell carcinoma RT-PCR IHC |
基金项目:中山大学2010年本科生业余科研Ⅰ项目(1163203) |
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中文摘要: |
摘 要:[目的] 探讨PAR1 mRNA及蛋白在食管鳞状细胞癌及其匹配癌旁组织中的表达和临床意义。[方法] 应用RT-PCR方法检测35例食管鳞状细胞癌患者食管原位癌组织及其匹配癌旁组织PAR1 mRNA的表达水平,并分析其与患者性别、年龄、肿瘤大小、临床分期及是否淋巴结转移等临床指标的关系;应用免疫组织化学法检测55对食管鳞癌组织及其匹配癌旁组织的PAR1蛋白表达情况。[结果] 食管鳞癌组织PAR1 mRNA的表达水平显著高于其匹配的癌旁组织(146.220±24.790 vs 134.054±23.593,t=-4.17,P<0.01),而且与淋巴结是否转移有关(t=-2.199,P<0.05);食管鳞癌组织PAR1蛋白表达阳性率显著高于其匹配的癌旁组织(49% vs 3.64%,χ2=23.04,P<0.001)。[结论] PAR1 mRNA及蛋白在食管鳞癌中的表达均显著上调,可能参与食管癌的发生发展、侵袭与转移。 |
英文摘要: |
Abstract:[Purpose] To explore PAR1 mRNA and protein expressions in esophageal squamous cell carcinoma(ESCC) tissues and their matched cancer adjacent tissues. [Methods] The expressions of PAR1 mRNA and protein in ESCC tissues and their matched cancer adjacent tissues were detected by RT-PCR and IHC, respectively. Meanwhile, the different expressions of PAR1 mRNA in ESCC tissues between different clinicopathologic features were analyzed.[Results] The expression level of PAR1 mRNA in ESCC tissues was significantly higher than that in their matched cancer adjacent tissues(146.220±24.790 vs 134.054±23.593,t=-4.17, P<0.01). ESCC patients with lymph node metastasis had a higher expression level of PAR1 mRNA(t=-2.199, P<0.05). Also, there was a higher positive rate of PAR1 protein expression in ESCC tissues(49% vs 3.64%,χ2=23.04,P<0.001). [Conclusion] Both PAR1 mRNA and protein expressions are upregu-lated in ESCC tissues, which might closely associate with carcinogenesis and progress of ESCC. |
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