| 张 宇,陈信义.贝母甲素抑制AML-KG-1a细胞增殖与诱导细胞凋亡研究[J].中国肿瘤,2015,24(4):325-329. |
| 贝母甲素抑制AML-KG-1a细胞增殖与诱导细胞凋亡研究 |
| Research of Verticine Inhibition of AML-KG-1 Cell Proliferation and Induction Cell Apoptosis |
| 投稿时间：2014-09-29 |
| DOI：10.11735/j.issn.1004-0242.2015.04.A015 |
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| 中文关键词: 贝母甲素 KG-1a细胞 凋亡 细胞周期 增殖抑制 |
| 英文关键词:Verticine KG-1a cell line apoptosis cell cycle proliferation inhibition |
| 基金项目:教育部高等学校博士点专项基金（20100013110008）； |
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| 中文摘要: |
| 摘 要：［目的］ 通过体外培养和流式细胞术，检测贝母甲素对人急性髓系白血病细胞KG-1a增殖抑制、诱导凋亡和细胞周期分布的影响。［方法］ 利用MTT法检测贝母甲素对KG-1a的增殖抑制作用，计算抑制率；以流式细胞术检测贝母甲素对细胞凋亡及周期分布的影响。［结果］ ①与对照组比较，贝母甲素作用于KG-1a细胞24h、48h、72h的吸光度（OD）值均明显降低（P＜0.05），且细胞生长抑制率与药物浓度呈正相关。②与对照组比较，贝母甲素能够提高KG-1a细胞晚期凋亡及总凋亡率，差异均有统计学意义（P=0.003，P=0.001）。③贝母甲素干预KG-1a后，细胞周期发生明显变化，G0/G1期细胞比例降低、G2/M期细胞比例升高、凋亡率升高（P=0.001，P=0.002，P=0.046）。［结论］ 贝母甲素具有抑制具有LSC特性的KG-1a细胞增殖、降低G0/G1期细胞比例、升高G2/M期细胞比例、诱导细胞凋亡效应。 |
| 英文摘要: |
| Abstract：［Purpose］ To detect the influence of Verticine on human acute myeloid leukemia cell KG-1a in inhibition proliferation，induction apoptosis and cell cycles by flow cytometry in vitro. ［Methods］ The effect and ratio of inhibition proliferation were conducted by MTT method. Cell apoptosis and the cell cycle distribution were detected by flow cytometry. ［Results］ (1) Compared with the control group，the absorbance(OD) values of KG-1a cells at 24 h，48 h and 72 h in Verticine group decreased(P<0.05). The inhibition rate of cell growth was positively correlated with increased drug concentration.(2) Verticine improved the late apoptosis and apoptotic rate of KG-1a cells，which were significantly different from control group(P=0.003，0.007). (3)After Verticine intervention，the cell cycle of KG-1a cells changed significantly：the value of G0/G1 phase declining，along with G2/M phase and cell apoptosis rate increasing(P=0.001，0.002，and 0.046). ［Conclusion］ Verticine can inhibit LSC KG-1a cell proliferation，enhance the G2/M phase ratio，decrease G0/G1 phase ratio and induce cell apoptosis. |
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