| 朱赴东,周 霞,陶雪芬.告达庭激活Caspase-3诱导人胃癌细胞SGC-7901凋亡[J].中国肿瘤,2014,23(6):523-527. |
| 告达庭激活Caspase-3诱导人胃癌细胞SGC-7901凋亡 |
| Caudatin Induced Apoptosis of Gastric Cancer Cell Line SGC-7901 by Activation of Caspase-3 |
| 投稿时间：2014-01-15 |
| DOI：10.11735/j.issn.1004-0242.2014.06.A017 |
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| 中文关键词: 告达庭 胃癌 凋亡 Caspase-3 SGC-7901 |
| 英文关键词:Caudatin gastric cancer apoptosis Caspase-3 SGC-7901 |
| 基金项目:浙江省自然基金项目（LQ12H28004）； |
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| 中文摘要: |
| 摘 要：［目的］ 评价告达庭体外诱导人胃癌细胞SGC-7901凋亡的作用。［方法］ MTT法检测细胞活性；吖啶橙染色观察核形态；流式细胞术检测细胞凋亡率；Western-blot及比色法检测Caspase-3表达及活性。［结果］ 告达庭抑制SGC-7901细胞的活性，具有浓度、时间依赖性；告达庭70μM能使SGC-7901细胞出现核染色体浓聚等典型的凋亡形态学改变，告达庭35、70μM使细胞凋亡率升高，并使Caspase-3蛋白表达量和蛋白活性显著升高。［结论］ 告达庭可能通过激活Caspase-3诱导SGC-7901细胞凋亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the apoptosis inducing effects and molecular mechanisms of Caudatin in gastric cancer cell SGC-7901. ［Methods］ SGC-7901 cells were cultured in vitro and treated with various concentrations of Caudatin. Cell proliferation was examined by MTT assay and apoptosis were detected by Flow cytometry. Morphological changes were detected by acridine orange staining. The protein expression of caspase-3 was detected by Western blot analysis and the activity of caspase-3 was detected by Colorimetric assay. ［Results］ Caudatin inhibited the growth of SGC-7901 cells in a time and concentration-dependent manner. Caudatin could also induce cell nuclear morphological changes，and increase the expression and activity of caspase-3.［Conclusion］ Caudatin may inhibit cell proliferation and induce the apoptosis of SGC-7901 by activating caspase-3. |
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