| 徐 洁,孙立新,遇 珑.单克隆抗体12H6的靶抗原在肺癌细胞中的表达及功能研究[J].中国肿瘤,2013,22(2):120-125. |
| 单克隆抗体12H6的靶抗原在肺癌细胞中的表达及功能研究 |
| Study on Expression and Function of Targeted Antigen of Anti-lung Cancer Monoclonal Antibody 12H6 |
| 投稿时间：2012-11-28 |
| DOI：10.11735/j.issn.1004-0242.2013.02.A2012345 |
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| 中文关键词: 肺癌 功能性单克隆抗体 靶向治疗 |
| 英文关键词:lung cancer functional monoclonal antibody targeted therapy |
| 基金项目:国家自然科学基金（81172033）；重大新药创制科技重大专项(2011ZX09102?鄄010?鄄02和2011ZX09401?鄄030) |
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| 中文摘要: |
| 摘 要: ［目的］ 分析抗肺癌单抗12H6的靶抗原在肺癌细胞系和肺癌组织中的表达，研究单抗12H6体内外生物学功能。［方法］ 细胞免疫荧光、流式细胞荧光法检测单抗12H6靶抗原在3种肺癌细胞系中的表达及其定位，免疫组化法检测其在人肺癌组织中表达及特异性；Transwell法和CCK-8法分别检测12H6体外对肺癌细胞系侵袭和增殖功能的影响。共接种模型裸鼠体内治疗实验研究12H6对移植瘤生长的抑制作用。［结果］ 单抗12H6的靶抗原在人肺腺癌细胞（A549、GLC-82）、人肺鳞癌细胞（GLC-P）的胞内及胞膜均有表达，在3种细胞系胞膜的表达比例分别为31.2%、15.4%和16.7%。单抗12H6在人肺癌组织中的表达比例为80.6%，较配对癌旁组织显著上调。单抗12H6在体外能明显抑制肺癌细胞侵袭和增殖；体内能明显抑制移植瘤生长，抑制率为40%。［结论］ 单抗12H6具有优良的体内外抑瘤功能，可能为肺癌靶向治疗提供潜在的靶向治疗药物。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the expression of the targeted antigen of anti-lung cancer monoclonal antibody 12H6 in lung cancer cell lines and lung cancer tissues，and to study the biological function of 12H6 mAb in vivo and in vitro.［Methods］ The expression and localization of targeted antigen in 3 lung cancer cell lines were examined by immunofluorescence and flow cytometry.Expression and specificity of 12H6 mAb in lung cancer tissues were examined by immunohistochemistry. Transwell and CCK-8 assay were performed to evaluate the function of 12H6 mAb against invasion and proliferation of lung cancer cell lines in vitro. Treatment experiment of co-inoculation model to nude mouse was performed to evaluate the inhibitory function of 12H6 mAb against the growth of transplantation tumor. ［Results］ The targeted antigen of 12H6 mAb were expressed in the cytoplasm and cell membrane of human lung adenocarcinoma cell lines(A549、GLC-82) and squamous carcinoma cell lines(GLC-P) . The expression rates at cell membrane of the 3 cell lines were 31.2%、15.4%、16.7% respectively. The expression of targeted antigen in lung cancer tissues was 80.6%， which was significantly up-regulated compared with paracancerious tissues. The 12H6 mAb obviously inhibited proliferation，invasion of lung cancer cells in vitro and growth of transplantation tumor in vivo with inhibitory rate of 40%.［Conclusions］ 12H6 mAb has excellent anti-tumor function in vivo and in vitro，and might be a potential agent in targeted therapy for lung cancer. |
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