| 翟宏芳,赵 刚,孙国贵.细丝蛋白A在肺鳞癌组织中的表达及临床意义[J].中国肿瘤,2013,22(2):130-133. |
| 细丝蛋白A在肺鳞癌组织中的表达及临床意义 |
| Expression of Filamin A in Lung Squamous Cell Carcinoma and Its Clinical Significance |
| 投稿时间：2012-10-22 |
| DOI：10.11735/j.issn.1004-0242.2013.02.A2012295 |
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| 中文关键词: 细丝蛋白A 肺肿瘤 逆转录聚合酶连反应 Western Blot |
| 英文关键词:filamin A lung squamous cell carcinoma RT-PCR Western Blot |
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| 中文摘要: |
| 摘 要: ［目的］ 探讨细丝蛋白A（filamin A，FLNa）在肺鳞癌组织中的表达及其临床意义。［方法］ 采用Western Blot方法和逆转录聚合酶链反应（RT-PCR），分别检测43例肺鳞癌组织及距肺鳞癌组织边缘5cm以上的镜下未见癌组织浸润的正常组织中FLNa蛋白、mRNA的表达情况。［结果］ FLNa蛋白在肺腺鳞组织及正常肺组织中的相对表达量分别为0.381±0.032、0.673±0.072，两组差异有统计学意义（P＜0.05）。FLNa mRNA在肺腺鳞组织及正常肺组织中的相对表达量分别为0.356±0.052、0.573±0.075，两组差异有统计学意义（P＜0.05）。FLNa蛋白、mRNA表达与肺鳞癌患者性别、年龄、肿瘤大小、肿瘤分化无关（P＞0.05）；而与淋巴结转移以及肿瘤TNM分期有关（P＜0.05）。［结论］ 肺鳞癌中FLNa 蛋白和mRNA的表达均降低，可能与肺鳞癌的发生、发展相关。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the expression of filamin A (FLNa) in lung squamous cell carinoma and its significance.［Methods］ The expression of FLNa protein and FLNa mRNA in 43 tissue specimens of lung adenocarcinoma and adjacent tissues 5cm outside the cancer without invasion in microscopically were detected by RT-PCR and Western ?注lot respectively. ［Results］ The relative expression of FLNa protein were 0.381±0.032 in lung squamous cell carcinoma，0.673±0.072 in cancer adjacent tissues with significant difference(P＜0.05). The relative expression of FLNa mRNA were 0.356±0.052 in lung squamous cell carcinoma and 0.573±0.075 in cancer adjacent tissues with significant difference(P＜0.05). FLNa expression did not correlate to gender，age，tumor size， tumor differentiation (P＞0.05)， but correlated to lymph node metastasis and TNM staging (P＜0.05). ［Conclusion］ The expression of FLNa protein and mRNA are both lower in lung squamous cell carcinoma than in normal tissues. FLNa might be correlate to oncogenesis and cancer progression. |
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