| ［Objective］To investigated the expression of microRNA-126 (miR-126), microRNA-155 (miR-155) and programmed death ligand 1 (PD-L1) in cervical cancer and its clinical significance.［Methods］From January 2020 to January 2022, 104 patients with cervical cancer who received surgical treatment in Henan Provincial People's Hospital and were confirmed by pathology were selected, and the surgically resected cervical cancer tissues and paracancerous tissue were collected. The expression of miR-126 and miR-155 was detected by fluorescence quantitative PCR, and the expression of PD-L1 was detected by immunohistochemistry. The relationship between the expression of miR-126, miR-155, PD-L1 and the clinicopathological characteristics and prognosis of cervical cancer patients was analyzed.［Results］①The levels of miR-126 expression in cervical cancer tissue was lower than that of paracancerous tissue, the levels of miR-155 and the positive rate of PD-L1 expression were higher than those of paracancerous tissue, and the differences were statistically significant (P<0.05). ②The proportion of patients with low miR-126 expression in International Federation of Gynecology and Obstetrics (FIGO) stage II, poorly differentiated, and lymph node metastasis is higher than that in patients with high miR-126 expression, and patients with high miR-155 expression in FIGO stage II, low The proportion of differentiation and lymph node metastasis was higher than that of patients with low expression of miR-155. PD-L1 positive patients with FIGO stage II, poorly differentiated and lymph node metastasis proportion were higher than PD-L1 negative patients, and the difference was statistically significant (P< 0.05). ③The proportion of FIGO stage II, poorly differentiated, and lymph node metastasis in the poor prognosis group was higher than that in the good prognosis group, and the low expression rate of miR-126 was higher than the prognosis In the good group, the high expression rate of miR-155 and the positive rate of PD-L1 were higher than those in the good prognosis group, and the difference was statistically significant (P<0.05). ④Cox regression analysis showed that: Low expression of miR-126 (HR=10.245, 95% CI=2.404~43.664), high expression of miR-155 (HR=6.292, 95% CI=1.875~21.121), positive PD-L1 (HR=3.588, 95% CI= 1.453-8.858), lymph node metastasis (HR=4.661, 95% CI= 1.984-10.951), low differentiation (HR=2.596, 95% CI= 1.151-5.858), FIGO stage II (HR=4.310, 95% CI=1.821~10.202) was a risk factor for poor prognosis in patients with cervical cancer (P<0.05).［Conclusion］The expression of miR-126, miR-155 and PD-L1 is related to FIGO stage, differentiation degree, lymph node metastasis and prognosis of cervical cancer. miR-126, miR-155 and PD-L1 may become molecular markers for diagnosis, treatment and evaluate prognosis. |
