| 孙 万,宋子阳,马 飞,等.多模态显影载药微球应用于肝肿瘤经动脉化疗栓塞的动物实验研究[J].肿瘤学杂志,2026,32(7):566-572. |
| 多模态显影载药微球应用于肝肿瘤经动脉化疗栓塞的动物实验研究 |
| Multimodal Imaging Drug-Loaded Microspheres for Transarterial Chemoembolization in a Rabbit Liver Cancer Model |
| 投稿时间：2025-12-04 |
| DOI：10.11735/j.issn.1671-170X.2026.07.B007 |
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| 中文关键词: 纳米组装微球 动脉栓塞术 肝肿瘤 动物实验 阿霉素 |
| 英文关键词:nano-assembled microspheres transarterial chemoembolization liver neoplasms animal experimentation Doxorubicin |
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| 中文摘要: |
| 摘 要：［目的］ 验证多模态显影纳米组装微球（nano-assembled microspheres，NAMs）在动物肝癌模型中的X线/CT/MR显影功能和经动脉化疗栓塞效果。［方法］ 基于前期研究，制备多模态显影纳米组装载药微球，并将其应用于兔肝肿瘤化疗栓塞研究。利用VX2瘤株构建兔肝癌模型。研究共成功纳入45只兔，实验分组：对照组15只（推注生理盐水）、空白微球组15只（推注空白微球）、阿霉素（ Doxorubicin，DOX）载药微球组15只［推注加载阿霉素的NAMs （Doxorubicin-nano assembled microspheres，DOX-NAMs）］。使用NAMs在数字减影血管造影（digital subtraction angiography，DSA）引导下行肝动脉栓塞术。通过术前/术后CT、MRI扫描和术中DSA，研究NAMs多模态显影功能。通过DSA、CT扫描和HE、Ki-67、TUNEL病理检查，研究负载阿霉素的NAMs动脉化疗栓塞效果。［结果］ 微导丝段定位针在兔肝内行CT扫描伪影影响范围较小，可作为肝内肿瘤标记；NAMs在兔VX2肝肿瘤血管和肿瘤内实现X线/CT/MR多模态下显影；利用DOX-NAMs经肝动脉栓塞，术后CT、MRI可见微球显影。病理结果显示相对于空白微球组和对照组，DOX-NAMs组肝内肿瘤坏死程度更高，化疗栓塞效果更好。［结论］ 多模态纳米组装载药微球这一新型载药栓塞微球在动物体内具备良好的多模态CT/MRI可视化显影能力，以及优异的动脉化疗栓塞效果和抗肿瘤效果，在介入栓塞应用中具有一定的优势和潜力。 |
| 英文摘要: |
| Abstract：［Objective］ To evaluate the X-ray/CT/MR imaging capability and transarterial chemoembolization efficacy of multimodal imaging nano-assembled microspheres(NAMs) in a rabbit liver cancer model. ［Methods］ Based on previous studies， multimodal imaging nano-assembled drug-loaded microspheres were prepared and applied to transarterial chemoembolization in rabbits with VX2 liver tumors. A total of 45 male New Zealand white rabbits were successfully included and divided into three groups (n=15 each)： control group (normal saline)， blank microsphere group (blank NAMs)， and Doxorubicin-NAMs group (DOX-loaded NAMs). Hepatic artery embolization was performed under digital subtraction angiography (DSA) guidance using NAMs. Multimodal imaging function was assessed via pre- and post-procedural CT， MRI， and intraoperative DSA. The chemoembolization efficacy of Doxorubicin-loaded NAMs (DOX-NAMs) was evaluated by DSA， CT， and histopathological examinations including HE， Ki-67， and TUNEL staining. ［Results］ The CT artifacts caused by the micro-guidewire segment (positioning needle) in the rabbit liver were minimal， allowing its use as an intrahepatic tumor marker. NAMs enabled multimodal X-ray/CT/MR imaging within the tumor vessels and parenchyma of rabbit VX2 liver tumors. Following transarterial embolization with DOX-NAMs， microsphere deposition was visualized on postoperative CT and MRI. Histopathological analysis revealed a higher degree of tumor necrosis in the DOX-NAMs group compared with the blank microsphere and control groups， indicating superior chemoembolization efficacy. ［Conclusion］ The novel multimodal nano-assembled drug-loaded microspheres exhibit excellent multimodal CT/MRI visualization， potent transarterial chemoembolization effect， and promising antitumor efficacy in vivo， demonstrating considerable advantages and potential for interventional embolization applications. |
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