| 刘 琦,张文茂,徐高生.MicroRNA-658对乳腺癌的抑制作用及机制分析[J].肿瘤学杂志,2025,31(9):776-783. |
| MicroRNA-658对乳腺癌的抑制作用及机制分析 |
| Inhibitory Effect of MicroRNA-658 on Proliferation and Biological Activities of Breast Cancer and Its Underlying Mechanism |
| 投稿时间：2024-09-05 |
| DOI：10.11735/j.issn.1671-170X.2025.09.B005 |
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| 中文关键词: 乳腺肿瘤 miRNA-658 增殖 自噬 迁移 侵袭 |
| 英文关键词:breast neoplasms miRNA-658 proliferation autophagy migration invasion |
| 基金项目:湖南省自然科学基金（2021JJ30694）；岳阳市科技局基础研发项目（2024031900008） |
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| 中文摘要: |
| 摘 要：［目的］ 观察微小RNA（microRNA，miRNA）-658在乳腺癌组织中的表达情况，分析其与乳腺癌临床特征的关系，并研究其对乳腺癌细胞增殖、迁移及侵袭的影响。［方法］ 收集2022年3月至2023年9月收治的28例经病理确诊为乳腺癌患者的癌组织样本和癌旁组织样本作为研究对象，采用逆转录定量聚合酶链反应检测miRNA-658在癌组织和癌旁组织的表达水平，以及miRNA-658在乳腺癌细胞（MDA-MB-231）和人乳腺上皮细胞（MCF-10A）中表达差异。采用慢病毒转染抑制miRNA-658在MDA-MB-231乳腺癌细胞株中的表达。采用细胞计数法、细胞小室迁移实验和划痕实验检测miRNA-658敲低对MDA-MB-231细胞增殖、迁移和侵袭的影响。采用蛋白质印迹实验检测上皮间质转化相关蛋白波形蛋白、E-钙黏蛋白、N-钙黏蛋白和自噬蛋白微管相关蛋白1轻链3（microtubule-associated protein 1 light chain 3，MAP1LC3）的表达。［结果］ 乳腺癌组织中的miRNA-658的表达量为3.11±3.74，在癌旁组织中的表达量为1.00±0.01，差异均有统计学意义（P＜0.05）。乳腺癌组织中的miRNA-658表达水平与患者年龄相关（P＜0.05）；与TNM分期、淋巴结转移、肿瘤直径、组织学分级和分子分型无关（P＞0.05）。MDA-MB-231中的miRNA-658表达量高于MCF-10A（P＜0.05）。慢病毒敲低miRNA-658的表达后，与对照组相比较，乳腺癌细胞的N-钙黏蛋白和波形蛋白的表达降低，E-钙黏蛋白和MAP1LC3的表达升高（P＜0.05）。抑制miRNA-658后，乳腺癌细胞的生长、迁移与侵袭能力减弱（P＜0.05）。［结论］乳腺癌组织和乳腺癌细胞中 miRNA-658处于高表达，且降低miRNA-658表达后，乳腺癌细胞自噬能力增强，增殖、迁移和侵袭的能力减弱。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the expression of microRNA-658 (miRNA-658) in breast cancer tissues， analyze its correlation with clinical characteristics and prognosis， and explore its effects on the proliferation， migration， and invasion of breast cancer cells. ［Methods］ Cancer tissues and adjacent normal tissues were collected from 28 patients diagnosed with breast cancer by biopsy between March 2022 and September 2023. The expression levels of miRNA-658 in tissues and in breast cancer MDA-MB-231 cell and human mammary epithelial MCF-10A cell were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Lentiviral transduction was used to knock down miRNA-658 expression in MDA-MB-231 cells. Cell proliferation， migration， and invasion were assessed by cell counting kit-8 (CCK-8) assay， transwell assay， and scratch wound healing assay， respectively. The protein expression levels of epithelial-mesenchymal transition (EMT)-related markers (Vimentin， E-cadherin， N-cadherin) and autophagy-related protein microtubule-associated protein 1 light chain 3(MAP1LC3) were detected by Western blot. ［Results］ The expression of miRNA-658 was significantly higher in breast cancer tissues (3.11±3.74) than that in adjacent normal tissues (1.00±0.01) (P<0.05). MiRNA-658 expression was correlated with patient age (P<0.05)， but not with TNM stage， lymph node metastasis， tumor size， histological grade， or molecular subtype (P>0.05). MDA-MB-231 cells showed higher miRNA-658 expression than MCF-10A cells (P<0.05). Knockdown of miRNA-658 led to decreased expression of N-cadherin and Vimentin， increased expression of E-cadherin and MAP1LC3， and inhibited proliferation， migration， and invasion of breast cancer cells (P<0.05). ［Conclusion］ miRNA-658 is highly expressed in breast cancer tissues and cells. Its knockdown enhances autophagy and suppresses proliferation， migration， and invasion of breast cancer cells. |
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