| 谢桂丹,黄婷婷,廖雪霞,等.胰腺癌患者血浆LINC01268和miR-582-5p的表达水平及其临床意义[J].肿瘤学杂志,2025,31(2):152-156. |
| 胰腺癌患者血浆LINC01268和miR-582-5p的表达水平及其临床意义 |
| Plasma LINC01268 and MiR-582-5p Levels in Patients with Pancreatic Cancer and Their Clinical Significance |
| 投稿时间：2024-07-22 |
| DOI：10.11735/j.issn.1671-170X.2025.02.B010 |
|
 |
| 中文关键词: 胰腺肿瘤 LINC01268 miR-582-5p 诊断 |
| 英文关键词:pancreatic neoplasms LINC01268 miR-582-5p diagnosis |
| 基金项目:海南省医药卫生科研项目（21A200074） |
|
| 摘要点击次数: 0 |
| 全文下载次数: 0 |
| 中文摘要: |
| 摘 要： ［目的］ 探讨LINC01268及miR-582-5p在胰腺癌中的表达水平及其诊断早期胰腺癌的价值。［方法］ 选取2022年1月至2024年3月儋州市人民医院收治的胰腺癌患者107例（胰腺癌组）、胰腺炎患者80例（良性组）和体检正常者50人（对照组）作为研究对象，107例胰腺癌患者分为31例早期胰腺癌和76例中晚期胰腺癌。荧光定量PCR技术检测LINC01268及miR-582-5p水平，化学发光法测定糖链抗原199（carbohydrate antigen 199，CA199）水平。应用多因素Logistic回归分析影响中晚期胰腺癌发生的危险因素，绘制受试者工作特征（receiver operating characteristic，ROC）曲线分析LINC01268、miR-582-5p及CA199水平对早期胰腺癌的诊断价值。 ［结果］ 胰腺癌组血浆LINC01268及CA199水平均明显高于良性组和对照组，而胰腺癌组血浆miR-582-5p水平明显低于良性组和对照组（P<均0.001）。与早期胰腺癌组比较，中晚期胰腺癌组血浆LINC01268水平明显升高（1.92±0.87 vs 3.60±1.28，P<0.001），而miR-582-5p水平明显降低（0.68±0.14 vs 0.24±0.05，P<0.001）；有淋巴结转移、 低分化以及CA199阳性组LINC01268水平明显升高，而miR-582-5p水平明显降低（P均<0.05）。多因素Logistic回归分析显示，LINC01268（OR=4.105，95%CI：2.983～12.864，P<0.001）及CA199（OR=2.790，95%CI：2.307～10.116，P=0.013）水平升高、miR-582-5p水平降低（OR=2.476，95%CI：1.915～9.521，P=0.005）是中晚期胰腺癌的危险因素。ROC曲线分析显示，LINC01268≥2.16、miR-582-5p≤0.57及CA199≥64.82 U/mL三者联合检测对早期胰腺癌诊断的曲线下面积（area under the curve，AUC）最大为0.925（95%CI：0.862～0.990），准确率为87.6%。［结论］ 胰腺癌患者血浆LINC01268表达升高，miR-582-5p表达降低，且与肿瘤分期、淋巴结转移等有关，LINC01268、miR-582-5p及CA199联合检测对早期胰腺癌具有较好的诊断价值。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate plasma LINC01268 and miR-582-5p levels in patients with pancreatic cancer and their clinical significance. ［Methods］ One hundred and seven patients with pancreatic cancer (PC group)， 80 patients with pancreatitis (benign group) admitted to Danzhou People’s Hospital from January 2022 to March 2024， and 50 healthy subjects (control group) were enrolled in the study. Among 107 PC patients， 31 cases with early stage and 76 cases with intermediate and advanced disease. The plasma levels of LINC01268 and miR-582-5p were detected by fluorescence quantitative RT-PCR， and plasma CA199 levels were detected by chemiluminescence method. The risk factors of advanced pancreatic cancer was analyzed by multivariate Logistic regression， and the diagnostic value of LINC01268， miR-582-5p and CA199 levels in diagnosis of intermediate/advanced pancreatic cancer was analyzed by receiver operating characteristic (ROC) curve. ［Results］ The levels of plasma LINC01268 and CA199 in PC group were significantly higher than those in benign group and control group， while the plasma level of miR-582-5p was significantly lower than those in benign and control groups (all P<0.001). Compared with the early pancreatic cancer group， the level of LINC01268 in the intermediate/advanced group was significantly higher (1.92±0.87 vs 3.60±1.28， P<0.001)， while the level of miR-582-5p was significantly lower (0.68±0.14 vs 0.24±0.05， P<0.001). The levels of LINC01268 were significantly increased in PC patients with lymph node metastasis， low differentiation and positive CA199， while the levels of miR-582-5p were significantly decreased (all P<0.05). Multivariate Logistic regression analysis showed that increased levels of LINC01268 (OR=4.105， 95%CI： 2.983～12.864， P<0.001) and CA199 (OR=2.790， 95%CI： 2.307～10.116， P=0.013)， and decreased levels of miR-582-5p (OR=2.476， 95%CI： 1.915～9.521， P=0.005) were risk factors for intermediate/advanced pancreatic cancer. When LINC01268≥2.16， miR-582-5p≤0.57 and CA199≥64.82 U/mL in combination the area under ROC curve (AUC) for the diagnosis of intermetiate/advanced pancreatic cancer was 0.925 (95%CI： 0.862～0.990)， and its accuracy was 87.6%. ［Conclusion］ The expression of LINC01268 in plasma increased and the expression of miR-582-5p decreased in pancreatic cancer patients， which is related to tumor stage and lymph node metastasis. The combined detection of LINC01268， miR-582-5p and CA199 has good diagnostic value for early/advanced pancreatic cancer. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |