| 尤泓杰,王雍博,刘郅涵,等.MicroRNA-183通过结合LRIG1促进膀胱癌疾病进展的研究[J].肿瘤学杂志,2024,30(12):1023-1030. |
| MicroRNA-183通过结合LRIG1促进膀胱癌疾病进展的研究 |
| MicroRNA-183 Promotes Malignant Features of Bladder Cancer Cells Through Targeting LRIG1 |
| 投稿时间：2024-08-15 |
| DOI：10.11735/j.issn.1671-170X.2024.12.B007 |
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| 中文关键词: 膀胱肿瘤 微小RNA-183 亮氨酸丰富重复免疫球蛋白样域蛋白1 增殖 迁移 侵袭 凋亡 |
| 英文关键词:bladder neoplasms microRNA-183 leucine-rich repeats and immunoglobulin-like domains 1 proliferation migration invasion apoptosis |
| 基金项目:浙江省医药卫生科技计划项目（2021KY988） |
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| 中文摘要: |
| 浙江省医药卫生科技计划项目（2021KY988）摘 要：［目的］ 探讨微小RNA-183（microRNA-183，miR-183）对膀胱癌（bladder cancer，BCa）疾病进展的影响及作用机制。［方法］ 体外培养人BCa细胞株T24，分别/联合抑制细胞的miR-183和亮氨酸丰富重复免疫球蛋白样域蛋白1（leucine-rich repeats and immunoglobulin-like domains 1，LRIG1）的表达，将细胞分为对照（normal control，NC）、miR-183 inhibior、shLRIG1和miR-183 inhibitor+shLRIG1四组，qRT-PCR检测miR-183和LRIG1 mRNA的表达，Western blot检测细胞中LRIG1和表皮生长因子受体（epidermal growth factor receptor，EGFR）的蛋白表达水平；CCK-8、划痕实验、Transwell法及流式细胞术分别检测细胞增殖、迁移、侵袭及凋亡情况。［结果］ 与NC组相比，miR-183敲减（miR-183 inhibior组）的T24细胞中LRIG1的表达增加而EGFR的表达降低（P<0.05），shLRIG1组中LRIG1表达降低而EGFR表达增加（P<0.05），shLRIG1+miR-183 inhibitor组逆转了单个因素敲减所导致的上述变化。此外，miR-183敲减后，T24细胞的增殖、迁移、侵袭水平降低而凋亡水平增加（P<0.05），shLRIG1组细胞的增殖、迁移、侵袭水平增加而凋亡水平降低（P<0.05），shLRIG1+miR-183 inhibitor组与NC组比较各项指标差异均无统计学意义。［结论］ miR-183可能通过靶向结合LRIG1来调控膀胱癌细胞EGFR的表达，进而影响了膀胱癌细胞的生物学功能。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the effect and mechanism of microRNA-183 (miR-183) on biological features of bladder cancer (BCa) cells. ［Methods］ The exprssions of miR-183 and leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) were knocked down in human bladder cancer T24 cells. T24 cells were divided into 4 groups： NC group， miR-183 inhibior group， shLRIG1 group and miR-183 inhibitor+shLRIG1 group. The miR-183 and LRIG1 mRNA expressions were detected by qRT-PCR. The protein expressions of LRIGI and epidermal growth factor receptor (EGFR) were detected by Western blot. The cell proliferation， cell migration， cell invasion and apoptosis were detected by CCK-8， Scratch test， Transwell assay and flow cytometry， respectively. ［Results］ Compared with NC group， LRIG1 protein expression significantly increased and EGFR expression significantly decreased in the miR-183 inhibitor group (P<0.05)， while LRIG1 protein expression significantly decreased and EGFR expression significantly increased in the shLRIG1 group (P<0.05)； the shLRIG1+miR-183 inhibitor reversed the above changes. The cell proliferation， migration， invasion decreased， and the apoptosis increased in the miR-183 inhibitor group (P<0.05)， while the cell proliferation， migration， and invasion increased， and the apoptosis decreased in the shLRIG1 group (P<0.05). There was no significant difference in the biological functions between the NC group and the shLRIG1+miR-183 inhibitor group. ［Conclusion］ MiR-183 may affect EGFR expression and regulate the bio-logical functions of bladder cancer cells through targeting LRIG1. |
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