| 梁秋然,秦亚斌,张阿润,等.肠道菌群与肺结节恶性程度相关性高通量测序关联研究[J].肿瘤学杂志,2024,30(12):1006-1013. |
| 肠道菌群与肺结节恶性程度相关性高通量测序关联研究 |
| Association of Gut Microbiota with the Malignant Degree of Pulmonary Nodules |
| 投稿时间：2024-07-12 |
| DOI：10.11735/j.issn.1671-170X.2024.12.B005 |
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| 中文关键词: 肺结节 肺肿瘤 恶性程度 肠道菌群 高通量测序 |
| 英文关键词:lung nodules lung neoplasms malignancy degree gut microbiota high-throughput sequencing |
| 基金项目:国家青年自然科学基金项目（82104957）；北京中医药“十四五”重点专科规划项目（BJZKBC0003） |
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| 中文摘要: |
| 摘 要：［目的］ 探究肺结节与肠道菌群的关系。［方法］ 采用前瞻性临床观察研究方法，对2022年1月1日至2022年12月31日期间在北京中医药大学东方医院肿瘤科和胸外科就诊的肺结节患者进行肺结节恶性程度诊断及其肠道菌群相关分析。应用16S rDNA测序方法对肺结节患者的肠道菌群进行检测，采用SPSS 20.0版软件对数据进行统计分析。［结果］ 共纳入54例肺结节患者，分为低危型肺结节组24例，高危型肺结节组16例和肺癌组14例。Sob’s指数及Rank-abundance曲线显示3组样本的肠道菌群具有较高的丰富度和均匀度，但肺癌组肠道菌群较其他两组多样性相对较低。不同性质的肺结节，其肠道菌群的优势菌种在属的水平相同，但是其相对丰度不同，粪杆菌属、双歧杆菌属随肺结节恶性程度增加占比下降（P均<0.05），而经黏液真杆菌属、拟杆菌属占比呈上升趋势（P均<0.05）。克氏秩和检验表明，3组患者肠道菌群属水平差异有统计学意义（P<0.05），Student’s-t检验进行两两比较表明肺结节恶性程度不同，其肠道菌群属水平亦存在差异（P<0.05）。［结论］ 不同恶性程度的肺结节患者肠道菌群存在差异，肠道菌群可作为辅助诊断肺结节性质的客观生物学依据。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the association of gut microbiota with the malignant degree of pulmonary nodules. ［Methods］ A total of 54 patients with pulmonary nodules diagnosed in Beijing University of Chinese Medicine from January 1st to December 31st， 2022 were enrolled， including 24 cases of low-risk lung nodules group， 16 cases of high-risk lung nodules and 14 cases of lung cancer. The intestinal microbiota was examined by the 16S rDNA sequencing method， and data were analyzed with the SPSS 20.0 software. ［Results］ The Sob’s index and Rank-abundance curves indicated that the gut microbiota of the three groups were rich and uniform， but the lung cancer group showed relatively lower diversity compared to the other two groups. The dominant bacterial genera in the gut microbiota were the same among three groups， but their relative abundances varied. The proportions of Faecalibacterium and Bifidobacterium decreased with the increased malignancy of lung nodules (all P<0.05) ， while the proportions of Blautia and Bacteroides increased (all P<0.05). The Kruskal-Wallis test revealed significant differences at the genus level of gut microbiota among the three groups (P<0.05)， and the Student’s t-test for pairwise comparisons showed that there were significant differences at the genus level of gut microbiota lung nodules with different malignancy degrees (P<0.05). ［Conclusion］ Gut microbiota differ with varying degrees of lung nodule malignancy， which may serve as an objective biological basis to assist in the diagnosis of the nature of lung nodules. |
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