| 王盈盈,耿建磊,刘文博,等.PRELP在消化道恶性肿瘤中的表达及与预后的相关性[J].肿瘤学杂志,2024,30(10):840-846. |
| PRELP在消化道恶性肿瘤中的表达及与预后的相关性 |
| Expression of Purine-Arginine-Rich and Leucine-Rich Protein in Gastrointestinal Malignant Tumors and Its Correlation with Prognosis Based on Bioinformatic Analysis |
| 投稿时间：2024-03-14 |
| DOI：10.11735/j.issn.1671-170X.2024.10.B007 |
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| 中文关键词: 脯氨酸/富亮氨酸末端富亮氨酸重复序列蛋白 消化道肿瘤 预后 |
| 英文关键词:purine-arginine-rich and leucine-rich protein gastrointestinal neoplasms prognosis |
| 基金项目:河北省科技厅重点研发计划（22377701D）；河北医科大学“十四五”临床医学创新研究团队支持计划（2022LCTD-A13）；河北省2022年政府资助省级医学优秀人才项目（冀财预复〔2022〕180 号）；河北省2023年度医学科学研究课题（20230123）；2022年度河北省医学适用技术跟踪项目（GZ2022044） |
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| 中文摘要: |
| 摘 要：［目的］ 观察脯氨酸/富亮氨酸末端富亮氨酸重复序列蛋白（purine-arginine-rich and leucine-rich protein，PRELP）在消化道恶性肿瘤中的表达及与预后的相关性，并探讨其在肿瘤微环境中与免疫浸润、免疫调节的关系。［方法］ 从癌症基因组图谱（The Cancer Genome Atlas，TCGA）数据库和GTEx数据库获取PRELP在4种消化道肿瘤（食管癌、胃腺癌、结肠腺癌和直肠腺癌）中的RNA表达数据，并分析PRELP差异表达情况。分析PRELP与肿瘤预后的相关性并绘制Kaplan-Meier生存曲线。使用STRING数据库进行蛋白互作分析。对差异基因进行功能富集分析，探索生物功能和潜在通路。使用CIBERSORT计算4种癌症免疫细胞丰度，并对其与PRELP表达进行相关性分析。应用皮尔森相关性检验计算PRELP基因表达与免疫检查点基因表达相关性。通过qRT-PCR验证PRELP在不同消化道肿瘤临床样本中癌组织与癌旁组织的差异表达。［结果］ PRELP在4种消化道肿瘤中的表达均低于正常组织（P<0.001）。PRELP高表达与胃腺癌、结肠腺癌、直肠腺癌的不良预后有关（P均<0.05），而其低表达则与食管癌的不良预后有关（P<0.05）。PRELP水平与肿瘤免疫功能、免疫细胞丰度及免疫检查点表达水平相关。此外，qRT-PCR证实PRELP在癌组织与癌旁组织间的表达存在显著差异。［结论］ PRELP与消化道恶性肿瘤临床预后相关，并在肿瘤微环境中与免疫浸润、免疫检查点表达呈显著相关性，PRELP可能可作为多种消化道肿瘤潜在的新的生物标志物和治疗靶点。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the expression of purine-arginine-rich and leucine-rich protein (PRELP) in gastrointestinal malignant tumors and explore its correlation with prognosis of patients. ［Methods］ The RNA expression data of PRELP in four gastrointestinal malignant tumors (esophageal cancer， stomach adenocarcinoma， colon adenocarcinoma and rectal adenocarcinoma) were obtained from The Cancer Genome Atlas (TCGA) and GTEx database， and then the differential expression of PRELP were analyzed. The correlation between PRELP expression and tumor prognosis was analyzed with Kaplan-Meier survival curve. Protein-protein interaction analysis was performed using the STRING database. Functional enrichment analysis of differentially expressed genes was conducted to explore biological function and potential pathways. Immune cell abundance was calculated for four tumors by CIBERSORT and the association with PRELP expression was performed. Pearson correlation test was applied to analyze the correlation between PRELP and immune checkpoint gene expression. The differential expression of PRELP in cancer and adjacent tissues in different gastrointestinal malignant tumors was verified by qRT-PCR. ［Results］ The expression of PRELP was significantly lower in four gastrointestinal malignant tumors than that in normal tissues (P<0.001)， and high PRELP expression was associated with poor prognosis in stomach adenocarcinoma， colon adenocarcinoma and rectal adenocarcinoma (all P<0.05)， whereas low PRELP expression was associated with poor prognosis in esophageal cancer (P<0.05). PRELP expression was associated with tumor immune function， immune cell abundance， and immune checkpoint expression. In addition， the difference of PRELP expression between cancerous and paracancerous tissues was confirmed by qRT-PCR results. ［Conclusion］ PRELP is related to clinical prognosis of gastrointestinal malignant tumors and shows a significant correlation with immune infiltration and immune checkpoint expression in the tumor microenvironment. PRELP may serve as a potential biomarker and therapeutic target for various gastrointestinal malignant tumors. |
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