| 王晓敏,郑 欣,何 静,等.Syncytin-1和Cripto-1在非小细胞肺癌患者胸腔积液外泌体中的表达及意义[J].肿瘤学杂志,2024,30(9):745-752. |
| Syncytin-1和Cripto-1在非小细胞肺癌患者胸腔积液外泌体中的表达及意义 |
| Expression of Syncytin-1 and Cripto-1 in Exosomes of Pleural Effusion in Non-Small Cell Lung Cancer and Their Clinical Significance |
| 投稿时间：2024-05-23 |
| DOI：10.11735/j.issn.1671-170X.2024.09.B006 |
|
 |
| 中文关键词: 非小细胞肺癌 外泌体 Syncytin-1 Cripto-1 呼吸道肿瘤标志物 |
| 英文关键词:non-small cell lung cancer exosome Syncytin-1 Cripto-1 respiratory tumor markers |
| 基金项目:山东省自然科学基金面上项目（ZR2020MH321） |
|
| 摘要点击次数: 95 |
| 全文下载次数: 53 |
| 中文摘要: |
| 摘 要：［目的］ 探讨Syncytin-1和 Cripto-1在非小细胞肺癌（non-small cell lung cancer，NSCLC）患者胸腔积液外泌体中的表达及其临床意义。［方法］ 对41例NSCLC和41例肺炎患者的胸腔积液进行外泌体的提取、鉴定、RNA提取及Syncytin-1、Cripto-1表达的检测。 通过蛋白印迹法检测外泌体蛋白表达，并利用酶联免疫吸附实验检测外泌体内蛋白含量，分析Syncytin-1和Cripto-1在NSCLC患者胸腔积液外泌体中的表达量与临床病理特征的相关性，并评估其对NSCLC的诊断效能。［结果］ 与肺炎患者相比，NSCLC患者的胸腔积液外泌体中Syncytin-1和Cripto-1的表达量升高（P<0.001）。蛋白印迹法检测显示外泌体表达其特异性蛋白。 Syncytin-1和Cripto-1在NSCLC患者组和肺炎患者组之间存在统计学差异（P<0.001）。Syncytin-1和Cripto-1表达量对NSCLC诊断灵敏度分别为82.9%和75.6%、特异度分别为75.6%和80.5%，曲线下面积（area under curve，AUC）分别为0.847和0.849。Syncytin-1和Cripto-1诊断NSCLC的AUC值高于神经元特异性烯醇化酶和胃泌素释放肽前体（AUC分别为0.762和0.716），但低于癌胚抗原（AUC=0.931）（P<0.001）。［结论］ 胸腔积液外泌体中Syncytin-1、Cripto-1检测对于诊断NSCLC具有较高的灵敏度和特异度，可作为辅助检测NSCLC的新型分子标志物。 |
| 英文摘要: |
| Abstract： ［Objective］ To explore the expression of Syncytin-1 and Cripto-1 in exosomes of pleural effusion in patients with non-small cell lung cancer(NSCLC) and their clinical significance. ［Methods］ The exosomes were extracted and identified from pleural effusion of 41 NSCLC and 41 pneumonia patients. The expression of Syncytin-1 and Cripto-1 in exosomes were detected with qRT-PCR. Western blotting was used to detect exosomal protein expression， and enzyme-linked immunosorbent assay (ELISA) was used to measure the contents of exoprotein. The association of Syncytin-1 and Cripto-1 expression in exosomes with clinicopathological features of NSCLC patients was analyzed， and the diagnostic efficacy of Syncytin-1 and Cripto-1 for NSCLC was evaluated. ［Results］ Compared with pneumonia patients， the expression levels of Syncytin-1 and Cripto-1 in exosomes of pleural effusion in NSCLC patients were significantly increased (P<0.001). Western blotting analysis detected specific proteins in exosomes. ELISA results showed that there were significant differences in the expression of Syncytin-1 and Cripto-1 in exosome between NSCLC patients and pneumonia patients(P<0.001). The area under the ROC curve(AUC) of Syncytin-1 and Cripto-1 for diagnosis of NSCLC were 0.847 and 0.849， with a sensitivity of 82.9% and 75.6%， specificity of 75.6% and 80.5%， respectively. The AUC values of Syncytin-1 and Cripto-1 were significantly higher than those of neuron-specific enolase and progastrin-releasing peptide (0.762 and 0.716)， but lower than that of carcinoembryonic antigen (AUC=0.931) (P<0.001). ［Conclusion］ The expression of Syncytin-1 and Cripto-1 in pleural effusion exosomes exhibits high sensitivity and specificity for diagnosis of NSCLC， which may be used as novel molecular markers for auxiliary diagnosis of NSCLC. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |