杨骐玮,黄聪玲,李 芳,等.黑蒜提取物通过调控PI3K/Akt/mTOR信号通路促进乳腺癌细胞凋亡[J].肿瘤学杂志,2024,30(7):551-555.
黑蒜提取物通过调控PI3K/Akt/mTOR信号通路促进乳腺癌细胞凋亡
Black Garlic Extract Promotes Apoptosis in Breast Cancer Cells Through PI3K/Akt/mTOR Pathway
投稿时间:2024-03-11  
DOI:10.11735/j.issn.1671-170X.2024.07.B004
中文关键词:  黑蒜提取物  乳腺癌细胞  增殖  凋亡
英文关键词:black garlic extract  breast cancer cells  proliferation  apoptosis
基金项目:中国金属学会冶金安全与健康分会健康卫生科研项目(jkws202042);内蒙古医科大学科技百万工程联合项目(YKD2020KJBW(LH)056)
作者单位
杨骐玮 内蒙古医科大学第三临床医学院 
黄聪玲 内蒙古医科大学第三临床医学院 
李 芳 内蒙古医科大学第三附属医院 
安 冉 内蒙古医科大学第三附属医院 
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中文摘要:
      摘 要:[目的] 探究不同浓度的黑蒜提取物(black garlic extract,BGE)对人乳腺癌细胞系-7(human breast cancer cell line-7,MCF-7)生长的影响,并进一步探讨其作用的机制。[方法] 通过应用噻唑蓝法(methyl thiazolyl tetrazolium,MTT)、5-溴脱氧尿嘧啶核苷(bromo-deoxyuridine,BrDU)法检测不同浓度(25、50、100 mg/mL)BGE对于乳腺癌细胞增殖的影响。应用流式细胞术(Annexin Ⅴ-FITC/PI双染色法)检测不同浓度BGE对于乳腺癌细胞凋亡的影响。应用蛋白质印迹法(Western blot)检测磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)、p-PI3K蛋白激酶B(Akt)、p-Akt、哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)、p-mTOR蛋白的表达。应用SPSS 26软件进行单因素方差分析。[结果] MTT结果表明,经不同浓度BGE作用后,24 h BGE 25、50、100 mg/mL实验组细胞增殖率(64.14%±1.52%,56.06%±3.12%,53.39%±1.62%)低于对照组(71.79%±2.73%)(P<0.01),48 h实验组细胞增殖率(81.23%±3.45%,74.71%±3.58%,69.42%±2.06%)低于对照组(89.28%±1.44%) (P<0.01),72 h实验组细胞增殖率(87.38%±3.65%,82.96%±8.46%,76.69%±6.60%)低于对照组(95.12%±3.98%)(P<0.01)。流式细胞术检测发现BGE 25、50、100 mg/mL实验组细胞凋亡率(9.57%±0.97%,15.23%±1.21%,17.42%±1.89%)高于对照组(5.53%±0.52%)(P<0.01)。 Western blot法检测发现实验组磷酸化的PI3K、Akt与mTOR蛋白的表达与对照组相比均受到抑制(P均<0.01)。 [结论] BGE对乳腺癌细胞增殖有抑制作用,对其凋亡有促进作用,其作用机制可能与PI3K/Akt/mTOR通路相关。
英文摘要:
      Abstract: [Objective] To investigate the effects and mechanism of black garlic extract (BGE) on the growth of human breast cancer cells. [Methods] Human breast cancer MCF-7 cells were treated with BGE at concentration of 25,50 and 100 mg/mL, respectively. The proliferation and apoptosis of MCF-7 cells were detected by MTT, BrDU and flow cytometry(Annexin Ⅴ-FITC/PI double staining) methods, respectively. The expression of phosphoinositide 3-kinase (PI3K), p-PI3K, protein kinase B (Akt), p-Akt, mammalian target of rapamycin (mTOR), and p-mTOR was detected by Western blot. One-way analysis of variance (ANOVA test) was applied with SPSS 26 software. [Results] MTT assay showed that the proliferation rate MCF-7 cells treated with BGE (25, 50 and 100 mg/mL) was lower than that of the control group (for 24 h: 64.14%±1.52%,56.06%±3.12% and 53.39%±1.62% vs 71.79%±2.73%, P<0.01; for 48 h: 81.23%±3.45%,74.71%±3.58% and 69.42%±2.06% vs 89.28%±1.44%, P<0.01; for 72 h: 87.38%±3.65%,82.96%±8.46% and 76.69%±6.60% vs 95.12%±3.98%, P<0.01). Flow cytometry showed that the apoptosis rate of the BGE groups was higher than that of the control group (for 48 h: 9.57%±0.97%, 15.23%±1.21% and 17.42%±1.89% vs 5.53%±0.52%, P<0.01). Western blot showed that expression of phosphorylated PI3K, Akt and mTOR proteins in the BGE groups were all inhibited compared with the control group (all P<0.01). [Conclusion] BGE may inhibit proliferation and promote apoptosis of breast cancer cell through the PI3K/Akt/mTOR pathway.
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