| 邹 燕,刘立民,蒙 杰,等.Circ_0001946靶向miR-671-5p调控Wnt/β-catenin通路对结直肠癌细胞恶性进展的影响[J].肿瘤学杂志,2024,30(6):458-466. |
| Circ_0001946靶向miR-671-5p调控Wnt/β-catenin通路对结直肠癌细胞恶性进展的影响 |
| Circ_0001946 Promotes Malignant Progression of Colorectal Cancer Cells by Activating Wnt/β-catenin Pathway Through miR-671-5p |
| 投稿时间：2023-07-07 |
| DOI：10.11735/j.issn.1671-170X.2024.06.B003 |
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| 中文关键词: circ_0001946 miR-671-5p 结直肠肿瘤 增殖 凋亡 |
| 英文关键词:circ_0001946 miR-671-5p colorectal neoplasms proliferation apoptosis |
| 基金项目:广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20190002，Z20190296，Z20200472)； 广西自然科学基金青年基金项目 (2019GXNSFBA185003)； 柳州市科学研究与技术开发计划课题 (2017BH20306)；广西高校中青年教师科研基础能力提升项目(2019KY0156) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨circ_0001946对结直肠癌细胞恶性进展的影响。［方法］培养结直肠癌HCT116、SW480、Colo320、HT29细胞和正常结直肠CCC-HIE-2细胞，实时定量PCR检测circ_0001946、miR-671-5p表达量。miRcode和双荧光素酶报告实验分析circ_0001946和miR-671-5p的关系。circ_0001946、miR-671-5p在HCT116细胞中的表达量被上调或下调后，分别检测HCT116细胞生长活性、细胞凋亡率及细胞迁移能力。免疫蛋白印迹法检测Bcl-2、Bax、Caspase-3和Caspase-9蛋白表达及Wnt/β-catenin信号通路蛋白表达。［结果］ HCT116（4.76±0.47）、HT29（2.85±0.57）、SW480（2.75±0.62）、Colo320（3.12±0.68）细胞中circ_0001946表达水平高于CCC-HIE-2细胞（1.24±0.09）（t=7.238、8.129、6.893、6.875，P均<0.05）。下调circ_0001946表达显著降低了HCT116细胞增殖（P<0.05）和迁移能力（P<0.05），促进了细胞凋亡（P<0.01）； circ_0001946靶向miR-671-5p； circ_0001946表达被上调或miR-671-5p表达被下调后，HCT116细胞增殖和迁移能力显著上升，细胞凋亡率明显降低，Wnt/β-catenin信号通路被激活。［结论］ circ_0001946靶向miR-671-5p激活了Wnt/β-catenin信号通路，促进了结直肠癌细胞恶性进展。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of circ_0001946 on the malignant progression of colorectal cancer cells and its mechanism. ［Methods］ The expression levels of circ_0001946 and miR-671-5p were detected in human colorectal carcinoma HCT116， SW480， Colo320， HT29 cells and normal colorectal CCC-HIE-2 cells by RT-qPCR. The relationship between circ_0001946 and miR-671-5p was analyzed by miRcode and dual luciferase reporting assay. After circ_0001946 and miR-671-5p expression in HCT116 cells were up-regulated or down-regulated， the growth activity， apoptosis rate and migration ability of HCT116 cells were detected respectively. The expression of Bcl-2， Bax， Caspase-3 and Caspase-9 and the proteins of Wnt/β-catenin signaling pathway were detected by Western blot. ［Results］ The expression levels of circ_0001946 in HCT116 (4.76±0.47)， HT29 (2.85±0.57)， SW480 (2.75±0.62)， and Colo320 (3.12±0.68) cells were significantly higher than those in CCC-HIE-2 cells(1.24±0.09)(t=7.238，8.129，6.893，6.875， all P<0.05). Down-regulation of circ_0001946 significantly reduced the proliferation (P<0.05) and migration (P<0.05) of HCT116 cells， and promoted cell apoptosis (P<0.01). The dual luciferase reporting assay showed that circ_0001946 targeted miR-671-5p. Up-regulation of circ_0001946 or down-regulation of miR-671-5p significantly enhanced proliferation and migration of HCT116 cells， decreased apoptosis rate， and activated Wnt/β-catenin signaling pathway. ［Conclusion］ The study indicates that circ_0001946 may promote malignant progression of colorectal cancer cells by activating the Wnt/β-catenin signaling pathway through miR-671-5p. |
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