| 尹子豪,张红芳,唐荣军,等.食管造影在诊断原位食管癌动物模型成功建立中的作用[J].肿瘤学杂志,2024,30(2):118-126. |
| 食管造影在诊断原位食管癌动物模型成功建立中的作用 |
| Application of Esophagography for Diagnosis of Esophageal Cancer in Established Mouse Model |
| 投稿时间：2023-11-01 |
| DOI：10.11735/j.issn.1671-170X.2024.02.B005 |
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| 中文关键词: 食管造影 食管癌原位动物模型 食管鳞癌 诊断 |
| 英文关键词:esophagography orthotopic esophageal cancer model esophageal squamous cell carcinoma diagnosis |
| 基金项目:浙江省医学会临床科研基金项目(2021ZYC-Z05)；杭州市农业与社会发展科研项目(2020ZDSJ0552)；“大医精诚”肿瘤防治研究及学术交流公益项目 |
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| 中文摘要: |
| 摘 要：［目的］ 评估食管造影在辅助诊断食管癌原位动物模型成功建立中的作用。［方法］ 采用饲喂致癌物4-硝基喹啉-1-氧化物(4-NQO)法建立C57BL/6小鼠食管鳞癌原位模型。28周后采用食管造影检查对模型小鼠的食管进行摄片并影像分析。分离获取模型小鼠的食管组织，采用HE染色及免疫组化法进行病理诊断，以评估食管造影的准确性及实用性。［结果］ 饲喂致癌物 4-NQO法成功建立了食管癌小鼠原位模型。成功对4只模型小鼠完成食管造影检查。4只模型小鼠的食管均呈现食管变粗、造影剂流通受阻及“充盈缺损”现象，提示了食管病变位置。通过HE染色及食管鳞癌标志物CK14免疫组化分析明确其中2只模型小鼠的食管病变为食管鳞癌，另外2只食管鳞状乳头状瘤，肿瘤显示率100%。［结论］ 食管造影可以辅助诊断小鼠食管癌原位模型的成功建立。 |
| 英文摘要: |
| Abstract：［Objective］ To evaluate the application of esophagography for the diagnosis of esophageal carcinoma in established animal model. ［Methods］ C57BL/6 mice were fed with carcinogen 4-nitroquinoline-1-oxide (4-NQO) to establish an orthotopic model of esophageal squamous cell carcinoma. After 28 weeks of modelling， the mice underwent esophagography； then the animals were sacrificed and the esophageal tissues were examined by HE staining and immunohistochemistry. ［Results］ An orthotopic mouse model of esophageal cancer was successfully established after 28 weeks of feeding 4-NQO. Esophagography was performed in 4 model mice， showing thickening of the esophagus， obstruction of the contrast agent flow and filling defect in the tumor lesions. HE staining and immunohistochemical CK14 staining confirmed the esophagographic diagnosis in 2 mice with esophageal squamous cell carcinoma， and other 2 mice with esophageal squamous papilloma. ［Conclusion］ Esophagography can assist the diagnosis in the establishment of orthotopic mouse esophageal cancer model. |
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