| 颜 波,常媛媛,杨晓华,等.肿瘤干细胞标志物与非小细胞肺癌患者生存的相关性分析[J].肿瘤学杂志,2023,29(11):934-941. |
| 肿瘤干细胞标志物与非小细胞肺癌患者生存的相关性分析 |
| Correlation Between Cancer Stem Cells Markers and Survival of Patients with Non-Small Cell Lung Cancer |
| 投稿时间：2023-05-09 |
| DOI：10.11735/j.issn.1671-170X.2023.11.B007 |
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| 中文关键词: 非小细胞肺癌 肿瘤干细胞 干细胞标志物 八聚体结合转录因子4 预后 |
| 英文关键词:non-small cell lung cancer cancer stem cells stem cell markers octamer-binding transcription factor 4 prognosis |
| 基金项目:国家自然科学基金资助项目（81773273）；上海市卫生健康委员会卫生行业临床研究专项（202140312） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨肿瘤干细胞（cancer stem cells，CSCs）标志物与非小细胞肺癌（non-small cell lung cancer，NSCLC）患者预后的关系。［方法］ 入组53例2017—2018年在上海市胸科医院进行手术并病理确诊的ⅢA期NSCLC患者，通过单因素和多因素回归方法分析临床因素与NSCLC患者总生存期（overall survival，OS）的相关性。通过免疫组化检测CSCs标志物（OCT4、SOX2、CD44、ALDH1A1）在NSCLC中的表达，并进行免疫组织化学评分。将CSCs标志物高H评分、年龄和微乳头成分6项指标进行累加评分，定义为肿瘤干性指数。利用Kaplan-Meier法分析肿瘤干性评分与NSCLC患者OS的关系。［结果］ 多因素回归分析结果显示年龄(HR=1.948，95%CI：1.092～2.474，P=0.031）、微乳头成分（HR=2.720，95%CI：1.267～5.842，P=0.011）和CEA水平（HR=1.008，95%CI：1.000~1.015，P=0.040）与OS 显著性相关。免疫组化结果显示，OCT4在肺腺癌和肺鳞癌中均有较高的表达。在肺腺癌中，OCT4高表达组预后较差（P=0.004）。SOX2、CD44、ALDH1A1对肺癌预后的影响不明显。肿瘤高干性评分与OS显著性相关(HR=2.212，95%CI：1.245~3.676，P=0.024)。［结论］在肺腺癌患者中，OCT4高表达和肿瘤高干性指数可能与预后差相关。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the correlation between cancer stem cells(CSCs) markers and the prognosis of non-small cell lung cancer(NSCLC) patients. ［Methods］ A total of 53 patients with stage ⅢA NSCLC who underwent surgery and were confirmed by pathology in Shanghai Chest Hospital from 2017 to 2018 were enrolled. The correlation between clinical factors and overall survival(OS) was analyzed by univariate and multivariate regression methods. Then， CSCs markers(OCT4， SOX2， CD44， ALDH1A1) were detected by immunohistochemistry(IHC) and scored. Cancer stem score(CSS) is accumulated by positive IHC markers， age(≥65 years-old) and micropapillary. Finally， the Kaplan-Meier method was used to analyze the correlation between the IHC scores of CSCs markers and the OS of NSCLC patients. ［Results］ The results of multivariate regression analysis showed that age (HR=1.948，95%CI：1.092～2.474， P=0.031) ， micropapillary pattern (HR=2.720， 95%CI：1.267~5.842， P=0.011) and CEA（HR=1.008， 95%CI：1.000~1.015， P=0.040） were significantly associated with OS. Immunohistochemical results showed that OCT4 was highly expressed in both lung adenocarcinoma and lung squamous cell carcinoma. In lung adenocarcinoma， OCT4 high expression group had a poor prognosis(P=0.004). SOX2， CD44 and ALDH1A1 had no significant effect on the prognosis of lung cancer. High CSS was significantly associated with OS(HR=2.212， 95%CI：1.245~3.676， P=0.024). ［Conclusion］ High expression of OCT4 and high CSS may be associated with poor prognosis in lung adenocarcinoma. |
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