| 杨文婧,郭珊珊,许丽萍,等.miR-221在110例喉鳞状细胞癌中的表达及其临床预后价值分析[J].肿瘤学杂志,2023,29(6):511-518. |
| miR-221在110例喉鳞状细胞癌中的表达及其临床预后价值分析 |
| Expression of miR-221 in 110 Cases with Laryngeal Squamous Cell Carcinoma and Its Prognostic Value |
| 投稿时间：2022-12-08 |
| DOI：10.11735/j.issn.1671-170X.2023.06.B010 |
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| 中文关键词: 喉鳞状细胞癌 miR-221 预后 增殖 侵袭 迁移 |
| 英文关键词:laryngeal squamous cell carcinoma miR-221 prognosis proliferation invasion migration |
| 基金项目:2021年度河北省医学科学研究课题计划项目（20211073） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨微小RNA-221（microRNA-221，miR-221）在喉鳞状细胞癌（laryngeal squamous cell carcinoma，LSCC）组织中的表达水平及其与临床病理特征和预后的关系。［方法］ 收集110例LSCC患者的临床病理资料，应用实时荧光聚合酶链反应（real-time fluorescence PCR，RT-PCR）方法检测LSCC组织中miR-221的表达。应用ROC曲线确定miR-221在LSCC癌组织中相对表达量作为最佳截断值，分析miR-221高低表达与LSCC患者临床病理特征的关系。应用Kaplan-Meier和Log-rank生存分析法分析miR-221表达与LSCC患者预后的关系。Nomogram列线图评估LSCC患者术后1、3、5生存率。免疫组化方法检测LSCC患者肿瘤组织中PD-L1的表达，分析PD-L1与miR-221表达的关系。［结果］ miR-221表达与LSCC患者的年龄有关（P＜0.05）。生存分析显示，miR-221低表达患者预后较高表达患者好（DFS：χ2=10.380，P=0.001 3；OS：χ2=7.356，P=0.006 7）。单因素和多因素分析结果显示：肿瘤大小、淋巴结转移、miR-221表达是LSCC患者无病生存期和总生存期的独立预后因素。miR-221低表达伴PD-L1高表达的LSCC患者，相对其他组生存时间长、预后好。［结论］ miR-221在LSCC组织中高表达，与患者不良预后有关；miR-221低表达伴PD-L1高表达者预后更好。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of microRNA-221(miR-221) in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinicopathological features and prognosis. ［Methods］ The clinicopathological data of 110 LSCC patients were collected. The expression of miR-221 in LSCC tissues was detected by real-time fluorescence polymerase chain reaction(qRT-PCR). The cut-off value of miR-221 expression was analyzed by ROC curve. The relationship of miR-221 expression with the clinicopathological features of LSCC patients was analyzed. The relationship of miR-221 expression with the prognosis of LSCC patients were determined by Kaplan-Meier method and Log-rank survival analysis. The efficiency of nomogram for predicting 1， 3 and 5-year survivals of LSCC patients was evaluated. The expression of PD-L1 in LSCC tissues was detected by immunohistochemistry， and the correlation between PD-L1 and miR-221 was analyzed. ［Results］ The expression level of miR-221 was associated with age(P<0.05). Survival analysis showed that patients with low expression of miR-221 had better prognosis than those with high expression (DFS： χ2=10.380， P=0.001 3； OS： χ2=7.356， P=0.006 7). The univariate and multivariate analysis showed that tumor size， lymph node metastasis and miR-221 expression were independent prognostic factors for disease-free survival and overall survival of LSCC patients. LSCC patients with low expression of miR-221 and high expression of PD-L1 had longer survival time and better prognosis. ［Conclusion］ The expression of miR-221 in LSCC tissue is related to the prognosis of patients， patients with low expression of miR-221 and high expression of PD-L1 would have better overall survival and disease-free survival. |
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