臧志意,魏小东.AHNAK2蛋白在肺腺癌中的表达及其对血管生成的影响[J].肿瘤学杂志,2022,28(10):818-825.
AHNAK2蛋白在肺腺癌中的表达及其对血管生成的影响
Expression of AHNAK2 in Lung Adenocarcinoma and Its Relation with Tumor Angiogenesis
投稿时间:2022-06-12  
DOI:10.11735/j.issn.1671-170X.2022.10.B004
中文关键词:  AHNAK2  肺癌  预后  血管生成
英文关键词:AHNAK2  lung cancer  prognosis  angiogenesis
基金项目:
作者单位
臧志意 中国人民解放军联勤保障部队第九〇四医院 
魏小东 中国人民解放军联勤保障部队第九〇四医院 
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中文摘要:
      摘 要:[目的]探讨AHNAK核蛋白2(AHNAK2)在肺腺癌中的表达及其对血管生成的影响。[方法]收集中国人民解放军联勤保障部队第九〇四医院2015年2月至2019年2月行肺癌根治术诊治的118例肺腺癌患者的组织蜡块及临床病理资料,分别利用免疫组化检测肺腺癌组织及配对癌旁组织中AHNAK2、Ki-67及CD34的表达情况,使用Western blot检测40例肺癌组织及其对应癌旁组织中AHNAK2蛋白相对表达情况。分析AHNAK2蛋白的表达水平与患者预后的关系以及对血管生成的影响。[结果] Western blot实验与免疫组化结果显示,AHNAK2蛋白在肺腺癌组织中的表达水平明显高于癌旁组织(P<0.05)。肺腺癌组织中AHNAK2蛋白的表达水平与Ki-67表达呈正相关(V=0.359,P<0.05),与MVD计数之间也呈正相关(rs=0.304,P<0.05)(P<0.05)。Cox单因素分析结果显示肿瘤组织分化程度、肿瘤直径、淋巴结转移、TNM分期以及AHNAK2蛋白表达水平是患者生存时间的影响因素(P均<0.05)。Cox多因素分析结果显示,AHNAK2高表达(HR=1.855,95%CI:1.092~3.149,P<0.05)与TNM分期(HR=2.802,95%CI:1.639~4.790,P<0.05)是影响患者生存的独立危险因素。[结论] AHNAK2蛋白在肺腺癌组织中高表达,与Ki-67及MVD计数呈正相关,且AHNAK2蛋白高表达是患者预后的独立危险因素,AHNAK2蛋白可能可作为提示患者预后不良的参考因素。
英文摘要:
      Abstract: [Objective] To investigate the expression of AHNAK2 in lung adenocarcinoma and its relation with tumor angiogenesis and the prognosis of patients. [Methods] The tissue wax blocks and clinicopathological data of 118 patients with lung adenocarcinoma who underwent radical mastectomy in the Department of Thoracic Surgery of The 904th Hospital of Joint Logistic Support Force of PLA from February 2015 to February 2019 were collected. The expressions of AHNAK2, Ki-67 and CD34 in lung adenocarcinoma tissues and paired adjacent tissues were detected by immunohistochemistry. The relative expression of AHNAK2 protein in 40 fresh lung cancer tissues and corresponding adjacent tissues were detected by Western blot. The relationship of the expression of AHNAK2 with tumor angiogenesis and the prognosis of patients were analyzed. [Results] The results of Western blot and immunohistochemistry showed that the expression level of AHNAK2 in lung adenocarcinoma tissues was significantly higher than that in adjacent tissues(P<0.05). The expression of AHNAK2 was positively correlated with the expression of Ki-67(V=0.359, P<0.05) and MVD count(rs=0.304, P<0.05) in lung adenocarcinoma tissue. Cox univariate analysis showed that tumor tissue differentiation, tumor diameter, lymph node metastasis, TNM stage and the expression level of AHNAK2 were significantly associated with the survival time of patients(all P<0.05). Cox multivariate analysis showed that high expression of AHNAK2(HR=1.855, 95%CI: 1.092~3.149, P<0.05) and TNM stage(HR=2.802, 95%CI: 1.639~4.790, P<0.05) were the independent risk factor for poor prognosis of patients. [Conclusion] AHNAK2 is highly expressed in lung adenocarcinoma tissues and has a positive correlation with Ki-67 and MVD counts. And the high expression of AHNAK2 is an independent risk factor for poor prognosis of patients, indicating that AHNAK2 may be used as a reference factor for prognosis of patients with lung adenocarcinoma.
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