| 李红乐,李 俊,郭兰伟,等.头颈部鳞癌患者HPV DNA、E6/E7 mRNA的分布及其在预后中的作用[J].肿瘤学杂志,2022,28(9):747-752. |
| 头颈部鳞癌患者HPV DNA、E6/E7 mRNA的分布及其在预后中的作用 |
| Detection of Human Papilloma Virus DNA and E6/E7 mRNA in Patients with Head and Neck Squamous Cell Carcinoma and Their Relation with Prognosis |
| 投稿时间：2022-06-30 |
| DOI：10.11735/j.issn.1671-170X.2022.09.B008 |
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| 中文关键词: 头颈部肿瘤 人乳头瘤病毒 E6和E7信使RNA |
| 英文关键词:head and neck carcinoma human papillomavirus E6/E7 mRNA |
| 基金项目:河南省自然科学基金项目（222300420574）；河南省科技攻关项目（212102310738）；河南省医学科技攻关计划项目（SBG202101008，RKX202002007） |
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| 中文摘要: |
| 摘 要：［目的］ 检测头颈部鳞癌组织标本人乳头瘤病毒(HPV)DNA、E6/E7 mRNA感染情况，并分析HPV感染对预后的影响。［方法］ 收集2016—2018年头颈部鳞癌手术标本121例，采用MassARRAY?誖HPV基因分型技术和RT-qPCR方法分别进行HPV DNA和HPV E6/E7 mRNA检测，比较不同肿瘤部位检出率差异。采用多因素Cox比例风险回归模型分析HPV E6/E7 mRNA对头颈部鳞癌预后的影响，计算HR值及其95%可信区间。［结果］ 121例头颈部鳞癌患者HPV阳性率为66.94%（81/121），其中口腔癌阳性率为70.37%（19/27），喉癌为65.79%（50/76），口咽癌为66.67%（12/18），HPV DNA阳性率在不同肿瘤部位无统计学差异（P>0.05）。HPV16阳性率为4.13%（5/121），HPV18阳性率为6.61%（8/121）。头颈部鳞癌HPV E6/E7 mRNA阳性率为28.93%（35/121），HPV E6/E7 mRNA阳性率在不同肿瘤部位中无统计学差异（P>0.05）。多因素分析结果显示，与HPV E6/E7 mRNA阴性组相比，HPV E6/E7 mRNA阳性组预后较好（HR=0.13，95%CI：0.04~0.46）。［结论］ 头颈部鳞癌患者HPV DNA阳性率高，但HPV E6/E7 mRNA阳性率明显降低。HPV感染为头颈部鳞癌独立的预后因素。 |
| 英文摘要: |
| Abstract： ［Objective］ To detect human papilloma virus（HPV） DNA and E6/E7 mRNA in head and neck squamous cell carcinoma(HNSCC)， and their relation with the prognosis of patients. ［Methods］ Surgical specimens were collected from 121 patients with HNSCC from 2016 to 2018. The MassARRAY?誖HPV genotyping was used to detect HPV DNA and RT-qPCR was used to detect HPV E6/E7 mRNA， respectively. The detection rates of different cancer sites were compared. Multivariate Cox proportional hazards regression model was used to analyze the association of HPV E6/E7 mRNA expression with the prognosis of HNSCC patients， and the hazard ratio with 95% confidence interval were calculated. ［Results］ The overall positive rate of HPV DNA in 121 HNSCC patients was 66.94%(81/121)， among which 70.37%(19/27) in oral cancer， 65.79%(50/76) in laryngeal cancer and 66.67%(12/18) in oropharyngeal cancer， the distribution was not statistical significance(P>0.05). The positive rate of HPV16 and 18 was 4.13%(5/121) and 6.61%(8/121)， respectively. The overall detection rate of HPV E6/E7 mRNA in HNSCC was 28.93%(35/121)， among which 33.33%(9/27) in oral cancer， 26.32%(20/76) in laryngeal cancer and 33.33%(6/18) in oropharyngeal cancer， the distribution was not statistical significance(P>0.05). Multivariate analysis showed that HNSCC patients with positive HPV E6/E7 mRNA had a better survival than those with negativeHPV E6/E7 mRNA(HR=0.13， 95%CI： 0.04~0.46). ［Conclusion］ The positive rate of HPV DNA in HNSCC patients is high， while the positive rate of E6/E7 mRNA is decreased significantly. HPV infection is an independent prognostic factor for HNSCC. |
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