| 芦文萱,唐伏秋,吴子熹,等.WP1130通过下调β-catenin增强鼻咽癌细胞化疗敏感性[J].肿瘤学杂志,2022,28(8):664-671. |
| WP1130通过下调β-catenin增强鼻咽癌细胞化疗敏感性 |
| WP1130 Enhances Chemosensitivity by Downregulating β-catenin Expression in Nasopharyngeal Carcinoma Cells |
| 投稿时间：2021-12-06 |
| DOI：10.11735/j.issn.1671-170X.2022.08.B007 |
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| 中文关键词: 鼻咽癌 顺铂 WP1130 β-catenin 增殖 迁移 侵袭 |
| 英文关键词:nasopharyngeal carcinoma cisplatin WP1130 β-catenin proliferation migration invasion |
| 基金项目:湖北省卫生健康委科研项目（WJ2021M221）；武汉中青年医学骨干人才培养工程 |
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| 中文摘要: |
| 摘 要：［目的］探讨WP1130与顺铂联合用药对鼻咽癌细胞增殖和转移的影响及其可能机制。［方法］ CCK-8法检测鼻咽癌顺铂敏感细胞系HNE1和顺铂耐药细胞系HNE1/DDP的顺铂药物半抑制浓度（IC50），以及不同浓度WP1130对鼻咽癌细胞活力的影响；选取适当药物浓度处理细胞，两种鼻咽癌细胞系分别分为4组：空白对照组（DMSO）、DDP单药组（6 ?滋mol/L顺铂）、WP1130单药组（1.25 ?滋mol/L WP1130）以及DDP+WP1130联合用药组（6 ?滋mol/L顺铂+1.25 ?滋mol/L WP1130）。MTT法检测鼻咽癌细胞增殖能力；细胞划痕实验检测鼻咽癌细胞迁移能力；Transwell侵袭实验检测鼻咽癌细胞侵袭能力；RT-PCR和Western blot实验检测顺铂耐药HNE1/DDP与亲本HNE1鼻咽癌细胞中β-catenin的表达情况以及不同药物处理对β-catenin的表达的影响。［结果］ 耐药HNE1/DDP细胞比亲本HNE1细胞对顺铂的耐受性提高了近5.7倍（5.17±0.33 vs 29.44±2.62，P＜0.01）。β-catenin在HNE1/DDP细胞中的mRNA表达上调约3.4倍（1.00±0.26 vs 3.44±0.39，P＜0.01）。与空白对照组和单药组相比，顺铂+WP1130联合用药组细胞中β-catenin的表达显著下调（HNE1：1.00±0.06 vs 0.78±0.07 vs 0.32±0.05，P＜0.05；HNE1/DDP：1.01±0.05 vs 0.57±0.07 vs 0.39±0.07，P＜0.05），顺铂+WP1130联合用药组细胞增殖、迁移和侵袭能力均显著下降（P均＜0.01）。［结论］ WP1130与顺铂联合用药可以通过下调β-catenin的表达增强顺铂对细胞增殖、迁移和侵袭的抑制作用，从而增强鼻咽癌细胞的化疗敏感性。 |
| 英文摘要: |
| Abstract：［Objective］ To explore effect of WP1130 combined with cisplatin on proliferation and metastasis of nasopharyngeal carcinoma(NPC) cells and its possible mechanism. ［Methods］ The IC50 of human NPC HNE1 cells(sensitive to cisplatin) and HNE1/DDP cells(resistant to cisplatin) to cisplatin was detected with CCK-8 assay. The HNE1 and HNE1/DDP cells were treated with no drugs(control group)， 6 mol/L cisplatin(DDP group)， 1.25 mol/L WP1130(WP1130 group)， or 6 mol/L cisplatin plus 1.25 mol/L WP1130(DDP+WP1130 group)， respectively. The cell proliferation was detected with MTT assay； cell migration ability was detected with wound-healing assay； cell invasion ability was detected with Transwell invasion assay； the expression of β-catenin mRNA and protein was detected with RT-PCR and Western blot， respectively. ［Results］ The IC50 of HNE1/DDP cells to cisplatin was 5.7 fold(5.17±0.33 vs 29.44±2.62， P<0.01) higher than that of HNE1 cells. The mRNA expression of β-catenin in HNE1/DDP cells was increased by 3.4 fold(1.00±0.26 vs 3.44±0.39， P<0.01). Compared with the blank control group and single drug group， the expression of β-catenin decreased significantly in cisplatin +WP1130 group(HNE1： 1.00±0.06 vs 0.78±0.07 vs 0.32±0.05， P<0.05； HNE1/DDP：1.01±0.05 vs 0.57±0.07 vs 0.39±0.07， P<0.05)， the proliferation， migration and invasion ability of cells in cisplatin+WP1130 group were significantly decreased(all P<0.01). ［Conclusion］ WP1130 can enhance the inhibiting effect of cisplatin in cell proliferation， migration and invasion in drug-resistant NPC cells and this chemosensitivity-enhancing effect is associated with the down-regulating β-catenin expression in NPC cells. |
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