| 刘 丹,王晓芳,李 娜,等.AXL在小细胞肺癌中表达的生物信息学分析及相关性研究[J].肿瘤学杂志,2022,28(5):370-379. |
| AXL在小细胞肺癌中表达的生物信息学分析及相关性研究 |
| Correlation of AXL Expression in Small Cell Lung Cancer： A Study Based on Bioinformatics Analysis |
| 投稿时间：2021-11-05 |
| DOI：10.11735/j.issn.1671-170X.2022.05.B006 |
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| 中文关键词: 小细胞肺癌 AXL 生物信息学分析 |
| 英文关键词:small cell lung cancer anxexelekto bioinformatics analysis |
| 基金项目:河北省政府资助临床医学优秀人才课题（361007）；河北大学附属医院青年科研基金项目（2019Q004） |
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| 中文摘要: |
| 摘 要：［目的］ 分析小细胞肺癌（small cell lung cancer，SCLC）中AXL表达与SCLC临床病理特征的关系。［方法］ 利用公共数据库分析SCLC中AXL的表达特征，对与AXL有显著性相关的基因进行GO及KEGG富集分析；应用免疫组化检测SCLC组织中AXL、Vimentin、E-cadherin的表达。［结果］ CCLE数据库检索结果显示SCLC细胞系的AXL表达水平最低（P<0.05）；Oncomine数据库检索结果显示SCLC组织中AXL的表达明显低于正常肺组织（P<0.05）或与正常肺组织无统计学差异（P>0.05）。CCLE 数据库筛选出 5 255 个在 SCLC 中与 AXL 表达有相关性的基因，其中EMT相关基因 VIM、MMP-2、MMP-9、ZEB-1、ZEB-2 和 TWIST-1与AXL表达呈正相关（P<0.05），N-cadherin和E-cadherin与AXL 表达呈负相关（P<0.05）；免疫组化结果显示SCLC中AXL、Vimentin的表达与临床病理参数无相关性，E-cadherin表达与肿瘤分期显著性相关（P=0.001），且AXL与Vimentin、E-cadherin表达不存在线性关系。［结论］ AXL在SCLC中表达较低且与EMT相关基因相关，免疫组化结果显示AXL在SCLC组织中的表达水平低于正常肺组织，与Vimentin、E-cadherin的表达无直线影响关系。 |
| 英文摘要: |
| Abstract： ［Objective］ To analyze the correlation of anexelekto(AXL) expression with clinicopathological features of small cell lung cancer（SCLC) based on bioinformatic study. ［Methods］ The public database was used to analyze the expression of AXL in SCLC， and the enrichment analysis of AXL-related genes were performed with GO and KEGG databases. The expression of AXL， Vimentin and E-cadherin in 60 SCLC tissue samples was detected by immunohistochemistry. ［Results］ CCLE database analysis showed that the expression level of AXL in SCLC cell line was the lowest(P<0.05). Oncomine database analysis showed that AXL expression in SCLC tissue was lower than that in normal lung tissue(P<0.05). A total of 5 255 genes related to AXL expression in SCLC were screened from CCLE database， among which VIM， MMP-2， MMP-9， ZEB-1， ZEB-2 and TWIST-1 were positively correlated with AXL expression(P<0.05)， while N-cadherin and E-cadherin were negatively correlated with AXL expression(P<0.05). Immunohistochemical results showed that the expression of AXL and Vimentin in SCLC was not correlated with clinicopathological parameters， but the expression of E-cadherin was significantly correlated with tumor stage(P=0.001). AXL expression had no significant linear relationship with Vimentin and E-cadherin expression. ［Conclusion］ The expression of AXL in SCLC is down-regulated， and the expression level of AXL in SCLC is lower than that in normal lung tissue， and it has no significant linear relationship with the expression of Vimentin and E-cadherin. |
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