| 孟 然,郑秀霞,朱 磊.蒲公英萜醇调控ABHD11-AS1介导上皮间质转化对膀胱癌细胞侵袭转移的作用[J].肿瘤学杂志,2022,28(1):53-57. |
| 蒲公英萜醇调控ABHD11-AS1介导上皮间质转化对膀胱癌细胞侵袭转移的作用 |
| Effect of Dandelion Terpenol on Invasion and Metastasis of Bladder Cancer Cells Through Regulating Epithelial Mesenchymal Transition Mediated by ABHD11-AS1 |
| 投稿时间：2021-07-03 |
| DOI：10.11735/j.issn.1671-170X.2022.01.B010 |
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| 中文关键词: 蒲公英萜醇 膀胱癌 α/β水解酶域11反义RNA1 细胞侵袭 迁移 |
| 英文关键词:dandelion terpenol bladder cancer RNA1 of alpha/beta hydrolase domain 11 cell invasion migration |
| 基金项目:国家自然科学基金项目（81700719） |
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| 摘要点击次数: 935 |
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| 中文摘要: |
| 摘 要：［目的］ 探究蒲公英萜醇通过调控α/β水解酶域11反义RNA1（ABHD11-AS1）对膀胱癌细胞侵袭转移的作用，并分析相关机制。［方法］ 将对数生长期的人膀胱癌细胞系EJ细胞分为空白对照组（细胞不做特殊处理）、蒲公英萜醇组（给予蒲公英萜醇100 μmol/L处理24 h）、蒲公英萜醇-pcDNA组（转染pcDNA 24 h给予细胞蒲公英萜醇100 μmol/L处理24 h）、蒲公英萜醇-pcDNA-ABHD11-AS1组（转染pcDNA-ABHD11-AS1 24 h后细胞给予蒲公英萜醇100 μmol/L药物处理24 h）。实时荧光定量PCR法检测细胞ABHD11-AS1水平；细胞增殖-毒性8检测试剂盒（CCK-8）法检测细胞增殖；划痕实验、Transwell实验分别检测细胞迁移、侵袭能力；蛋白免疫印迹法检测上皮间质转化标志蛋白E-钙黏附蛋白（E-cadherin）、N-钙黏附蛋白（N-cadherin）、波形蛋白（Vimentin）表达变化。［结果］ 与空白对照组比较，蒲公英萜醇组EJ细胞ABHD11-AS1水平、侵袭细胞数、细胞迁移率、N-Cadherin、Vimentin蛋白水平均降低（P＜0.05），EJ细胞增殖抑制率、E-cadherin蛋白水平升高（P＜0.05）。与蒲公英萜醇组、蒲公英萜醇-pcDNA组比较，蒲公英萜醇-pcDNA-ABHD11-AS1组EJ细胞ABHD11-AS1水平、侵袭细胞数、细胞迁移率、N-Cadherin、Vimentin蛋白水平均升高（P＜0.05），EJ细胞增殖抑制率、E-cadherin蛋白水平降低（P＜0.05）。［结论］ 蒲公英萜醇能够抑制膀胱癌细胞侵袭及迁移，可能是通过抑制ABHD11-AS1介导的上皮间质转化发生实现的。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the effect of dandelion terpenol(DT) on invasion and migration of bladder cancer cells via regulating antisense RNA1 of alpha/beta hydrolase domain 11(ABHD11-AS1). ［Methods］ Human bladder cancer EJ cells were divided into blank control group， DT group， DT-pcDNA group and DT-pcDNA-ABHD11-AS1 group. DT group was treated with 100 μmol/L DT for 24 h， DT-pcDNA group was transfected with pcDNA， then treated with 100 μmol/L DT for 24 h， and DT-pcDNA-ABHD11-AS1 group was transfected with pcDNA-ABHD11-AS1， then treated with 100 μmol/L DT for 24 h. The levels of ABHD11-AS1 were detected by real-time fluorescent quantitative PCR， cell proliferation was detected by cell counting kit-8(CCK-8)， the ability of cell migration and invasion was detected by scratch test and Transwell test， the expression of E-cadherin， N-cadherin and Vimentin was detected by Western blot. ［Results］ Compared with blank control group， the ABHD11-AS1 level， cell invasion， cell migration， N-Cadherin and Vimentin protein levels in DT group were significantly decreased(P＜0.05)， the proliferation inhibition rate and E-cadherin protein level increased(P<0.05). Compared with DT group and DT-pcDNA group， the ABHD11-AS1 level， cell invasion， cell migration， N-Cadherin and Vimentin protein levels in DT-pcDNA-ABHD11-AS1 group were increased(P<0.05) ， the cell proliferation inhibition rate and E-cadherin protein level decreased(P<0.05). ［Conclusion］ Dandelion terpenol can inhibit the invasion and migration of bladder cancer cells， which may be associated with inhibition of lncRNA ABHD11-AS1-mediated EMT. |
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