| 刘 丹,韩贵良,霍 悦.血浆proGRP和I-TAC蛋白在小细胞肺癌中的意义[J].肿瘤学杂志,2022,28(1):19-27. |
| 血浆proGRP和I-TAC蛋白在小细胞肺癌中的意义 |
| Potential Value of Plasma proGRP and I-TAC Proteins in Small Cell Lung Cancer |
| 投稿时间：2021-09-18 |
| DOI：10.11735/j.issn.1671-170X.2022.01.B005 |
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| 中文关键词: 小细胞肺癌 肿瘤标志物 胃泌素释放肽前体 干扰素诱导T细胞趋化因子 |
| 英文关键词:small cell lung cancer tumor markers progastrin releasing peptide interferon-inducible T cell alphachemoattractant |
| 基金项目:河北省政府资助临床医学优秀人才课题（361007）；河北大学附属医院青年科研基金项目（2017Q021；2019Q004） |
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| 中文摘要: |
| 摘 要: ［目的］ 分析血浆样本中的差异蛋白在小细胞肺癌（small cell lung cancer，SCLC）诊断及预后中的价值。［方法］ 首先应用GSH-CAA-X00蛋白芯片技术检测4例SCLC治疗前后及4名健康人外周血，结合生物信息学分析筛选差异蛋白。应用ELISA检测36例SCLC治疗前后及8名健康人血浆样本中关键差异蛋白胃泌素释放肽前体(progastrin releasing peptide，proGRP)、干扰素诱导T细胞趋化因子（interferon-inducible T cell alphachemoattractant，I-TAC）的表达，分析其表达与SCLC临床指征之间的关系。［结果］蛋白芯片结果显示在健康人和 SCLC 患者治疗前血浆样本中符合筛选且有统计学意义的差异蛋白有 PDGF-AB、S100A8、GHR、GP73、GRO、proGRP、I-TAC等，在 SCLC 患者治疗前后血浆样本中符合筛选且有统计学意义的差异蛋白有 MMP-3、NSE、LRIG3、proGRP、I-TAC等。PDGF-AB、S100A8、GHR、GP73、GRO、MMP-3、NSE、proGRP、I-TAC在健康人、SCLC患者治疗前、治疗后的表达趋势为先上升后下降，其中proGRP、I-TAC在两组间的变化最为显著且都具有统计学意义。ELISA结果显示proGRP、I-TAC与芯片结果一致，即与健康人相比，在SCLC患者表达升高，经治疗后下降，但与临床指征无相关性。［结论］ 差异蛋白尤其是proGRP、I-TAC可能是SCLC的潜在诊治肿瘤标志物，单独使用或与其他常用生物标志物联合使用可能有助于SCLC的早期诊断及预后判断。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate plasma proteins with potential value for diagnosis and prognosis of small cell lung cancer(SCLC). ［Methods］ The differentially expressed proteins were detected with GSH-CAA-X00 protein chip and analyzed with bioinformatics from plasma samples of 4 cases of SCLC before and after treatment， and 4 healthy subjects. The key differential proteins progastrin releasing peptide(proGRP) and interferon-inducible T cell alpha chemoattractant（I-TAC）were detected with ELISA in plasma samples of 36 SCLC patients before and after treatment and 8 healthy subjects. ［Results］ The proteins differentially expressed between SCLC patients before treatment and healthy subjects were PDGF-AB， S100A8，GHR，GP73，GRO，proGRP，I-TAC，etc. The proteins differentially expressed in SCLC patients before and after treatment were MMP-3，NSE，LRIG3，I-TAC，etc. Compared with healthy controls， the plasma levels of PDGF-AB，S100A8，GHR，GP73，GRO，MMP-3，NSE，LRIG3，proGRP，I-TAC in SCLC patients were significantly increased， then decreased after treatment， among which the changes of proGRP and I-TAC were the most significant. ELISA results showed that the changes of proGRP and I-TAC were consistent with the chip results， however， which were not correlated with clinical indications. ［Conclusion］ Differentially expressed proteins in plasma， especially proGRP and I-TAC， may be used as potential tumor markers for diagnosis and prognosis of SCLC. |
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