| 查艳梅,张军玉,於 薇.TNF-α通过NF-κB信号通路调控宣威肺癌恶性生物学行为[J].肿瘤学杂志,2021,27(7):560-565. |
| TNF-α通过NF-κB信号通路调控宣威肺癌恶性生物学行为 |
| TNF-α Regulates Malignant Biological Behavior of Xuanwei Lung Cancer Through NF-κB Signal Pathway |
| 投稿时间：2020-09-21 |
| DOI：10.11735/j.issn.1671-170X.2021.07.B009 |
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| 中文关键词: TNF-α NF-κB信号通路 宣威肺癌 增殖 凋亡 细胞周期 迁移 |
| 英文关键词:TNF-α NF-κB signal pathway Xuanwei lung cancer proliferation apoptosis cell cycle migration |
| 基金项目:云南省教育厅科学研究基金教师类项目（2020J1253） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨肿瘤坏死因子-α（TNF-α）通过影响核因子-κB（NF-κB）信号通路对宣威肺癌恶性生物学行为的调控。［方法］ ELISA检测临床收集宣威肺癌、非宣威肺癌及正常人血清样本中TNF-α水平。RT-qPCR、Western blot检测人支气管上皮样细胞16HBE、人肺腺癌细胞A549及宣威肺癌细胞XWLC-05中TNF-α；CCK-8检测细胞增殖水平；流式细胞术检测细胞凋亡率及细胞周期；Transwell检测细胞迁移能力；Western blot检测NF-κB经典及非经典信号通路相关蛋白水平。［结果］ TNF-α在宣威肺癌患者血清及细胞中显著性高表达，差异均有统计学意义（P<0.05）。沉默TNF-α不仅阻滞细胞周期、抑制细胞增殖及迁移，并促进凋亡（P<0.05）；也抑制NF-κB经典信号通路。XWLC-05被外源性TNF-α促进的恶性生物学行为可被NF-κB信号通路抑制剂扭转（P<0.05）。［结论］ TNF-α通过激活NF-κB经典信号通路促进宣威肺癌细胞恶性生物学行为。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the regulation of tumor necrosis factor-α(TNF-α) on malignant biological behavior of Xuanwei lung cancer by influencing nuclear factor-κB(NF-κB) signal pathway. ［Methods］ Serum samples of Xuanwei lung cancer， lung cancer and normal people were collected， then the level of TNF-α was detected by ELISA. RT-qPCR and Western blot was used to detect the expression of TNF-α mRNA and protein in normal human bronchial epithelioid cell line 16HBE， human lung adenocarcinoma cell line A549 and Xuanwei lung cancer cell line XWLC-05. Cell proliferation， apoptosis rate， cell cycle and migration were measured by CCK-8， flow cytometry and transwell assay. Moreover， the expression of protein of classical and noncanonical NF-κB signal pathway related proteins were detected by Western blot. ［Results］ TNF-α was highly expressed in serum and cell line of Xuanwei lung cancer(P<0.05)， silencing TNF-α significantly blocked the cell cycle， inhibited cell proliferation and migration， and promoted cell apoptosis(P<0.05). In addition， silencing TNF-α markedly inhibited the classical NF-κB signal pathway. Further experiments confirmed that NF-κB signal pathway inhibitor reversed the promotion of malignant biological behavior of Xuanwei lung cancer which was caused by TNF-α(P<0.05). ［Conclusion］ TNF-α promotes the malignant biological behavior of Xuanwei lung cancer cells by activating the classical NF-κB signal pathway. |
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