| 吴友军,秦 俭.结直肠癌合并2型糖尿病患者MAPK通路相关靶点表达差异及对预后的影响[J].肿瘤学杂志,2021,27(4):288-293. |
| 结直肠癌合并2型糖尿病患者MAPK通路相关靶点表达差异及对预后的影响 |
| Expression of MAPK Pathway Related Proteins in Patients with Colorectal Cancer Complicated with Type 2 Diabetes Mellitus and Its Impact on Prognosis |
| 投稿时间：2020-10-15 |
| DOI：10.11735/j.issn.1671-170X.2021.04.B010 |
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| 中文关键词: 2型糖尿病 结直肠肿瘤 信号通路 预后 |
| 英文关键词:type 2 diabetes mellitus colorectal cancer pathway Prognosis |
| 基金项目:广西自然科学基金(2016GXNSFAA380106) |
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| 摘要点击次数: 939 |
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| 中文摘要: |
| 摘 要：［目的］ 探究ERK1/2、JNK、SNX1及miR-95在结直肠癌(colorectal cancer，CRC)合并2型糖尿病（type 2 diabetes mellitus，T2DM）患者肿瘤组织中的表达情况及与预后的关系。［方法］ 回顾性分析71例CRC以及46例T2DM合并CRC患者病理标本，采用免疫组化和qRT-PCR法测定组织中ERK1/2、p-ERK1/2、JNK、p-JNK、SNX1和miR-95的表达及差异。采用卡方检验评价各靶点表达的差异，Kaplan-Meier法进行生存分析，Log-rank法分析各靶点不同表达情况下的生存差异。［结果］与CRC组比较，T2DM+CRC组中ERK1/2、p-ERK、JNK、p-JNK、miR-95表达上调，SNX1蛋白表达下调，差异均有统计学意义（P<0.05）；Ⅲ～Ⅳ期T2DM+CRC组的总生存（OS）及无进展生存（PFS）较CRC组差，差异有统计学意义（P<0.05）；ERK、JNK高磷酸化水平组患者的OS及PFS差于低水平组患者，SNX1低表达患者的OS及PFS差于高表达患者，miR-95高表达患者的PFS差于低表达患者，差异均有统计学意义（P<0.05）。［结论］ 合并T2DM的CRC患者预后不佳，ERK1/2、JNK通路及miR-95、SNX1可能参与调控CRC进程。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the expression of MAPK pathway related proteins and its prognosis significance in patients with colorectal cancer(CRC) complicated with type 2 diabetes mellitus(T2DM) . ［Methods］ The pathological specimens of 71 CRC patients(CRC group) and 46 T2DM with CRC patients(CRC+T2DM group) were collected. The expressions of ERK1/2，JNK，SNX1，miR-95，phosphorylation of ERK and JNK were detected by immunohistochemistry and qRT-PCR. The difference of protein expression levels in two groups were analyzed by Chi-square test. The survival of patients was analyzed with Kaplan-Meier curves and the difference of survival between two groups was analyzed by Log-rank test. ［Results］ Compared with CRC group，the expression level of ERK1/2，JNK and miR-95 was up-regulated in the T2DM+CRC group(P<0.05)，the phosphorylation level of ERK and JNK was also higher in the T2DM+ CRC group(P<0.05)，while the expression level of SNX1 was down-regulated(P<0.05). The overall survival(OS) and progression free survival(PFS) of T2DM + CRC group were worse than those of CRC group(P<0.05) in stage Ⅲ～Ⅳ stage patients. The OS and PFS in patients with high ERK and JNK phosphorylation were worse than those with low phosphorylation；the OS and PFS in patients with low expression of SNX1 were worse than those with high expression，and in high expression of miR-95 were worse than those with low expression(all P<0.05). ［Conclusion］ The prognosis of CRC patients with T2DM is poorer. ERK1/2，JNK pathway，miR-95 and SNX1 may be involved in the regulation of CRC process. |
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