| 姜 超,许 承,陈 霞.MicroRNA-1254靶向组蛋白去乙酰化酶7调控胃癌细胞增殖和侵袭[J].肿瘤学杂志,2021,27(4):255-259. |
| MicroRNA-1254靶向组蛋白去乙酰化酶7调控胃癌细胞增殖和侵袭 |
| MicroRNA-1254 Regulates Gastric Cancer Cell Proliferation and Invasion Through Targeting Histone Deacetylase 7 |
| 投稿时间：2020-08-24 |
| DOI：10.11735/j.issn.1671-170X.2021.04.B004 |
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| 中文关键词: MiR-1254 胃肿瘤 组蛋白去乙酰化酶7 增殖 侵袭 |
| 英文关键词:MiR-1254 gastric cancer histone deacetylase 7 proliferation invasion |
| 基金项目:国家临床重点专科普通外科项目（41732113） |
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| 中文摘要: |
| 摘 要：［目的］ 探究microRNA-1254（miR-1254）对胃癌细胞增殖、侵袭的调控作用及分子机制。［方法］ 使用TargetScan7.1工具预测分析miR-1254对组蛋白去乙酰化酶7（histone deacetylase 7，HDAC7）的靶向作用位点；miR-1254 mimics和miR-1254 inhibitor转染胃癌细胞系SGC-7901，用实时荧光定量PCR检测miR-1254及HDAC7 mRNA表达；CCK-8法检测胃癌细胞增殖活性；Transwell法检测胃癌细胞侵袭能力；蛋白质免疫印迹实验检测STAT3、磷酸化STAT3的表达水平。［结果］ MiR-1254可以靶向结合HDAC7 mRNA 3′UTR区域。与对照组相比，转染miR-1254 mimics组miR-1254表达水平升高（t=13.51，P=0.0002），HDAC7 mRNA表达水平显著性降低（t=4.667，P=0.0095），而转染miR-1254 inhibitor组miR-1254表达水平显著性降低（t=10.41，P=0.0005），HDAC7 mRNA表达水平显著性升高（t=4.008，P=0.016）。与对照组相比，转染miR-1254 mimics组细胞增殖活性显著性下降（F=30.4，P<0.0001），细胞侵袭能力显著性减弱（t=6.284，P=0.0033）；与转染miR-1254 mimics组相比，miR-1254 mimics+HDAC7组细胞增殖活性升高（F=22.16，P=0.0002），且侵袭能力增强（t=5.842，P=0.0043）。转染miR-1254 mimics抑制了STAT3的磷酸化，转染HDAC7表达载体可以减弱miR-1254对STAT3磷酸化的抑制作用。［结论］ MiR-1254可以通过靶向HDAC7抑制胃癌细胞STAT3的活化，并抑制细胞的增殖和侵袭。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects and molecular mechanism of microRNA-1254(miR-1254) on proliferation and invasion of gastric cancer cells. ［Methods］ The targeting site of miR-1254 on Histone Deacetylase 7(HDAC7) was analyzed with TargetScan 7.1 online tool. Gastric cancer cells SGC-7901 were transfected with miR-1254 mimics and miR-1254 inhibitors, the expression of miR-1254 and HDAC7 mRNA was analyzed by real-time qPCR. The proliferation rate of gastric cancer cells was measured by CCK-8 assay，cell invasion activities were measured by Transwell assays，expression and phosphorylation levels of STAT3 were detected by immunoblotting assays. The data were statistically analyzed by Graphpad Prim 8.0. ［Results］ MiR-1254 targeted on the 3′UTR region of HDAC7 mRNA. Compared with control group，the expression of miR-1254 was increased(t=13.51，P=0.0002) and HDAC7 mRNA was decreased(t=4.667，P=0.0095) in miR-1254 mimics group，while the expression of miR-1254 was decreased(t=10.41，P=0.0005) and HDAC7 mRNA was increased(t=4.008，P=0.016) in miR-1254 inhibitor group. Compared with control group，the cell proliferation(F=30.4，P<0.0001) and invasion activities(t=6.284，P=0.0033) were decreased in miR-1254 group; compared with miR-1254 group，the cell proliferation(F=22.16，P=0.0002) and invasion activities(t=5.842，P=0.0043) were decreased in miR-1254+HDAC7 group. Transfection of miR-1254 mimics inhibited STAT3 phosphorylation，and transfection of HDAC7 vectors reduced the inhibition of STAT3 phosphorylation by miR-1254.［Conclusion］ MiR-1254 inhibits the activation of STAT3 and the proliferation and invasion of gastric cancer cells through targeting HDAC7. |
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