| 普彦淞,薛 飞,都庆国.MiR-144-5p在胃癌中的表达及其生物学功能[J].肿瘤学杂志,2021,27(4):249-254. |
| MiR-144-5p在胃癌中的表达及其生物学功能 |
| Expression and Biological Function of MiR-144-5p in Gastric Cancer |
| 投稿时间：2020-10-26 |
| DOI：10.11735/j.issn.1671-170X.2021.04.B003 |
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| 中文关键词: 胃肿瘤 增殖 细胞周期 侵袭 miR-144-5p |
| 英文关键词:gastric carcinoma proliferation cell cycle invasion miR-144-5p |
| 基金项目:陕西省自然科学基础研究计划（2020JQ-947）；2017年西安市科技计划项目［2017113SF/YX007(11)］；2017年陕西省创新能力支撑计划（2017KCT-28） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-144-5p在胃癌组织和细胞中的表达及生物学功能。［方法］ 采用qRT-PCR 检测miR-144-5p在胃癌组织和细胞系中的表达水平，并验证miR-144-5p mimics的转染效率；分别采用CCK-8法、克隆形成实验、流式细胞仪、Transwell实验检测miR-144-5p mimics对胃癌细胞AGS和HGC-27增殖、克隆形成能力、细胞周期和侵袭能力的影响。［结果］ MiR-144-5p在胃癌组织的表达水平明显低于癌旁组织（0.607±0.257 vs 1.000±0.360，t=5.616，P<0.001）。MiR-144-5p低表达与胃癌患者的肿瘤浸润深度、淋巴结转移、远处转移、TNM分期显著相关（P<0.05）。MiR-144-5p在胃癌细胞SNU-1、HGC-27、SGC-7901、KATO Ⅲ及AGS中表达水平显著低于正常人胃黏膜上皮细胞GES-1（F=12.17，P<0.001）。转染miR-144-5p mimics后，AGS（t=4.902，P=0.001）和HGC-27（t=4.154，P=0.003）细胞中miR-144-5p的表达水平显著增高；AGS和HGC-27细胞的增殖能力、克隆形成能力、侵袭能力均被抑制（P<0.05），G1期细胞比例增加、而S期的细胞比例减少（P<0.05）。［结论］ MiR-144-5p在胃癌组织和细胞中表达降低，与胃癌患者的TNM分期较晚和胃癌细胞恶性程度较高有关，提示miR-144-5p在胃癌的恶性进程中起重要作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression and biological function of miR-144-5p in gastric cancer. ［Methods］The expression of miR-144-5p in gastric cancer tissues and gastric cancer cells was detected by qRT-PCR. Human gastric cancer AGS and HGC-27 cells were transfected with miR-144-5p mimics，and miR-144-5p expression in transfected cells was determined. The effect of up-regulation of miR-144-5p on the proliferative ability was examined by CCK-8 assay and colony formation assay. Cell cycle analysis and invasion of AGS and HGC-27 cells were detected by flow cytometry and Transwell assay，respectively. ［Results］ The expression level of miR-144-5p in gastric cancer tissues was significantly lower than that in adjacent tissues(0.607±0.257 vs 1.000±0.360，t=5.616，P<0.001). Low miR-144-5p expression was significantly correlated with depth of tumor invasion，lymph node metastasis，distant metastasis and TNM stage in patients with gastric cancer(P<0.001). The expression of miR-144-5p in gastric cancer cells(SNU-1，HGC-27，SGC-7901，KATO Ⅲ and AGS)was significantly lower than that in human normal gastric mucosal epithelium(F=12.17，P<0.001). The transfection of miR-144-5p mimics up-regulated miR-144-5p expression in AGS(t=4.902，P=0.001) and HGC-27 cells(t=4.154，P=0.003). Upregulation of miR-144-5p significantly decreased the proliferation，clone formation and invasion of HGC-27 and AGS cells，increased the proportion of cells in G1 phase，and decreased the proportion of cells in S phase(P<0.05). ［Conclusion］ The decreased expression of miR-144-5p in gastric cancer tissues and gastric cancer cells is associated with the advanced TNM stage and higher malignancy of gastric cancer cells，suggesting that miR-144-5p plays an important role in the malignant progression of gastric cancer. |
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