| 洪小山,侯明敏,文 斌.下调泛素结合酶E2异构体-1对子宫内膜癌细胞的增殖及凋亡的影响[J].肿瘤学杂志,2021,27(3):206-211. |
| 下调泛素结合酶E2异构体-1对子宫内膜癌细胞的增殖及凋亡的影响 |
| Effect of Down-regulation of UEV1 on Proliferation and Apoptosis of Endometrial Cancer Cells |
| 投稿时间：2020-04-14 |
| DOI：10.11735/j.issn.1671-170X.2021.03.B010 |
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| 中文关键词: 泛素结合酶E2异构体-1 子宫内膜癌 增殖 凋亡 |
| 英文关键词:ubiquitin binding enzyme E2 isomer-1 endometrial cancer proliferation apoptosis |
| 基金项目:广东省医学科学技术研基金项目(A2018059) |
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| 中文摘要: |
| 摘 要：［目的］ 研究下调泛素结合酶E2异构体-1(ubiquitin binding enzyme E2 isomer-1，UEV1)对子宫内膜癌细胞的增殖及凋亡的影响。［方法］ 经过细胞培养和细胞转染将Ishikawa细胞分为空白组、下调UEV1组及上调UEV1组。采用聚合酶链反应检测各组UEV1表达；细胞增殖检测各组细胞增殖能力；采用Transwell检测各组细胞侵袭；采用流式细胞仪检测各组细胞凋亡率；蛋白质印迹法检测各组活化转录因子6(activated transcription factor 6，ATF6)、C/EBP同源蛋白(C/EBP homologous protein，CHOP)、原癌基因(cell-myc，C-myc)及细胞增殖因子-67(Ki-67)蛋白相对表达。［结果］ 与空白组、上调UEV1组相比，下调UEV1组UEV1表达量降低(F=5.699，P＜0.001)，72h细胞增殖率降低(F=9.202、14.433、17.391，均P＜0.001)，72h细胞凋亡率增高(F=9.134、22.101、17.797，均P＜0.001)。与空白组、与上调UEV1组相比，下调UEV1组ATF6、Caspase-3表达量增高，C-myc、Ki-67表达量降低(F=7.888、6.971、11.233、10.129，均P＜0.001)。［结论］ 下调UEV1通过调控ATF6、CHOP、C-myc及Ki-67抑制子宫内膜癌细胞的增殖与侵袭，促进细胞凋亡，为子宫内膜癌的靶向治疗提供新的策略具有重要参考价值。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of down-regulation of ubiquitin binding enzyme E2 isomer-1(UEV1) on the proliferation and apoptosis of endometrial cancer cells. ［Methods］ Human endometrial carcinoma Ishikawa cells were transfected with UEV1 NC(blank group)，UEV1 mimic(UEV1 up-regulation group) or UEV1 inhibitor(UEV1 down-regulation group). The expression of UEV1 was quantitatively detected by PCR，cell proliferation was detected by the MTT method，the invasion of cells was detected by the Transwell assay，the expressions of activated transcription factor 6(ATF6)，C/EBP homolo-gous protein(CHOP)，proto-oncogene(C-myc) and cell proliferation factor-67(Ki-67) protein were detected by the Western blot. ［Results］ Compared with the blank group and the UEV1 up-regulation group，the expression of UEV1 in the UEV1 down-regulation group decreased(F=5.699，P＜0.001)，the proliferation rate decreased at 24，48 and 72h after culture(F=9.202，14.433，17.391，all P＜0.001) and cell apoptosis rate increased at 24，48 and 72h(F=9.134，22.101，17.797，all P＜0.001). Compared with the blank group and the UEV1 up-regulation group，the expression levels of ATF6 and Caspase-3 in the UEV1 down-regulation group increased，while the expression levels of C-myc and Ki-67 decreased(F=7.888， 6.971，11.233，10.129，all P＜0.001). ［Conclusion］ Down regulating UEV1 can inhibit the proliferation and invasion of endometrial cancer cells and promote cell apoptosis by regulating ATF6，chop，c-myc and Ki-67，which may provide new strategies for targeted treatment of endometrial cancer. |
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