| 宣 斌,郭运生,武 玉.MiR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响[J].肿瘤学杂志,2020,26(12):1056-1061. |
| MiR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响 |
| The Effect of MiR-655 on Chemotherapy Sensitivity of Colorectal Cancer Cells to Oxaliplatin |
| 投稿时间：2020-07-29 |
| DOI：10.11735/j.issn.1671-170X.2020.12.B010 |
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| 中文关键词: 结直肠肿瘤 miR-655 奥沙利铂 敏感性 |
| 英文关键词:colorectal cancer miR-655 oxaliplatin chemosensitivity |
| 基金项目:秦皇岛市科技支撑项目（201701B042） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响。［方法］ 通过qRT-PCR 验证奥沙利铂耐药和敏感结直肠癌组织蜡块中miR-655的相对表达量；CCK-8法检测细胞活性；Transwell检测细胞侵袭能力；流式细胞术检测细胞周期。［结果］ 相比于癌旁的正常组织，对奥沙利铂耐药的结直肠癌组织中miR-655表达量相对较低（1.24±0.28 vs 0.25±0.12，P<0.01），而对奥沙利铂敏感的结直肠癌组织中miR-655表达量相对较高（1.24±0.28 vs 2.68±0.76，P<0.01）。QRT-PCR鉴定miR-655转染效率结果显示，在miR-655 mimics组中miR-655表达水平相对于miR-NC组显著升高（1.70±0.10 vs 0.83±0.07，P=0.004）。CCK-8检测细胞活性结果显示，与L-OHP/miR-NC组相比，L-OHP/miR-655 mimics组细胞活性降低（48h：0.79±0.05 vs 0.56±0.03，P=0.0015；72h：0.65±0.05 vs 0.25±0.05，P=0.0081）。Transwell法检测细胞的侵袭能力结果显示，与L-OHP/miR-NC组相比，L-OHP/miR-655 mimic组细胞侵袭数量减少（845±85 vs 613±36，P=0.0292）。流式细胞术检测细胞周期结果显示，与L-OHP/miR-NC组G0/G1期相比，L-OHP/miR-655 mimics组细胞比例增加（5.16±0.22 vs 45.53±0.75，P=0.022），与L-OHP/miR-NC组S期相比，L-OHP/miR-655 mimics组细胞比例降低（44.83±1.17 vs 20.75±0.36，P=0.015），与L-OHP/miR-NC组G2/M期相比，L-OHP/miR-655 mimics组细胞比例减少(22.86±1.21 vs 3.85±0.35，P=0.0023）。［结论］MiR-655能够提高结直肠癌细胞对奥沙利铂的化疗敏感性，miR-655可能成为联同奥沙利铂治疗结直肠癌的新靶点。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the effect of miR-655 on chemosensitivity of colorectal cancer cells to oxaliplatin(L-OHP). ［Methods］ The relative expression of miR-655 in colorectal cancer tissue samples from 8 oxaliplatin-resistant patients and 8 oxaliplatin-sensitive patients was detected by qRT-PCR. Human colon cancer SW620 cells were transfected with miR-655 mimic(miR-655 mimics group) and blank vector(miR-NC group)，respectively. The viability，invasion ability and cell cycle of SW620 cells was detected by CCK-8 method，Transwell assay and flow cytometry，respectively. ［Results］ Compared with the adjacent normal tissues，the expression of miR-655 in oxaliplatin-resistant colorectal cancer tissues was decreased(1.24±0.28 vs 0.25±0.12，P<0.01)，while the expression of miR-655 in oxaliplatin-sensitive colorectal cancer tissues was increased(1.24±0.28 vs 2.68±0.76，P<0.01). The qRT-PCR test showed that the expression of miR-655 in miR-655 mimics group was significantly higher than that in miR-NC group(1.70±0.10 vs 0.83±0.07，P=0.004). CCK-8 assay showed that compared with L-OHP/miR-NC group，L-OHP/miR-655 mimics group had lower cell viability(48h：0.79±0.05 vs 0.56±0.03，P=0.0015；72h：0.65±0.05 vs 0.25±0.05，P=0.0081). Transwell assay showed that compared with L-OHP/miR-NC group，the invasion number of L-OHP/miR-655 mimic group was decreased(845±85 vs 613±36，P=0.0292). Compared with L-OHP /miR-NC group，the proportion of G0/G1 cells in L-OHP/miR-655 mimics group was increased(5.16±0.22 vs 45.53±0.75)，while the proportion of S phase and G2/M phase cells in L-OHP/miR-655 mimics group was decreased(44.83±1.17 vs 20.75±0.36，P=0.015；22.86±1.21 vs 3.85± 0.35，P=0.0023，respectively). ［Conclusion］ The expression of miR-655 in oxaliplatin-resistant colorectal cancer is down-regulated；and miR-655 can improve the sensitivity of colorectal cancer SW620 cells to oxaliplatin chemotherapy. |
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