| 尹 丽,杜鸣宇,樊琰鑫.KIF23在食管癌组织中的表达及临床价值[J].肿瘤学杂志,2020,26(12):1041-1046. |
| KIF23在食管癌组织中的表达及临床价值 |
| Expression of KIF23 in Esophageal Carcinoma and Its Clinical Significance |
| 投稿时间：2020-10-15 |
| DOI：10.11735/j.issn.1671-170X.2020.12.B007 |
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| 中文关键词: 食管肿瘤 KIF23 预后 |
| 英文关键词:esophageal carcinoma KIF23 prognosis |
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| 中文摘要: |
| 摘 要：［目的］ 探究驱动蛋白23（kinesin superfamily 23，KIF23）在食管癌组织中的表达水平，分析其与临床特性的相关性及对患者预后的影响，进一步探讨KIF23在食管癌细胞中的生物学功能。［方法］ 利用免疫组化方法检测70例食管癌患者癌组织及其癌旁组织中KIF23的表达；分析KIF23表达与临床病理因素的相关性；利用细胞学实验探究KIF23的生物学功能。［结果］ 食管癌组织中KIF23阳性表达率（57%）高于其对应的癌旁组织（11%）（P<0.01）。KIF23表达与临床分期和T分期密切相关(χ2=7.124，P=0.008；χ2=4.769，P=0.029)。生存分析显示高表达KIF23组患者的生存时间较低表达KIF23组明显缩短。COX回归单因素和多因素结果显示KIF23的表达水平是食管癌患者预后的独立危险因素（HR=4.902，95%CI：2.041～11.775，P<0.001；HR=6.372，95%CI：2.209～18.377，P=0.001）。细胞功能学实验表明沉默KIF23 能够显著抑制食管癌细胞的侵袭与迁移能力。［结论］ KIF23在食管癌组织中高表达，其表达与食管癌患者的肿瘤进程密切相关，可作为食管癌诊断和预后判断肿瘤标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of KIF23 in esophageal carcinoma and iits relation with clinicopathological features and prognosis of patients. ［Methods］ Immunohistochemical staining was used to detect the expression of KIF23 in cancer tissue and corresponding adjacent tissues of 70 patients with esophageal carcinoma. The correlation between KIF23 expression and clinicopathological features was analyzed. The KIF23 expression was knocked down in esophageal cancer Eca-109 and TE-1 cells and the cell biological functions were investigated. ［Results］ The positive expressions of KIF23 in EC tissues was higher than that in corresponding normal tissues(57% vs 11%，P<0.01). The expression of KIF23 was correlated with clinical stage and T stage(χ2=7.124，P=0.008. χ2=4.769，P=0.029). Kaplan-Meier analysis indicated that the overall survival(OS) of patients with low KIF23 expression was higher than that of patients with high KIF23 expression. Univariate and multivariate analysis showed that high expression of KIF23 was the independent prognostic factor for OS of patients(HR=4.902，95%CI：2.041～11.775，P<0.001. HR=6.372，95%CI：2.209～18.377，P=0.001). Transwell assays exhibited that knockdown of KIF23 expression significantly reduced the cell invasion and migration compared with the controls. ［Conclusion］ KIF23 is highly expressed in esophageal cancer tissues，which is closely related to the esophageal cancer tumor progression. KIF23 might be used as a tumor marker for the diagnosis and prognosis of patients with esophageal cancer. |
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