| 苏红娥,钟庆吉,符春丽.血清miR-153和miR-142-3p表达在宫颈癌诊断和预后评估中的价值[J].肿瘤学杂志,2020,26(11):972-976. |
| 血清miR-153和miR-142-3p表达在宫颈癌诊断和预后评估中的价值 |
| Value of Serum MicroRNA-153 and MicroRNA-142-3p in Diagnosis and Prognosis of Cervical Cancer |
| 投稿时间：2019-11-26 |
| DOI：10.11735/j.issn.1671-170X.2020.11.B009 |
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| 中文关键词: 宫颈癌 miR-153 miR-142-3p 诊断价值 预后评估 |
| 英文关键词:cervical cancer miR-153 miR-142-3p diagnostic value prognostic evaluation |
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| 中文摘要: |
| 摘 要：［目的］ 探讨宫颈癌患者血清miR-153及miR-142-3p的表达及其在宫颈癌诊断和预后评估中的价值。［方法］ 选取2014年1月至2017年3月收治的宫颈癌患者142例，采用实时荧光定量聚合酶链反应（RT-PCR）检测宫颈癌患者血清miR-153及miR-142-3p表达水平。绘制ROC曲线分析miR-153及miR-142-3p对宫颈癌诊断的截断值，并以此为标准将宫颈癌患者分为高表达和低表达，对两组临床病理特征进行比较。采用多因素Cox回归模型分析影响宫颈癌患者术后预后不良的危险因素。［结果］ 宫颈癌组血清miR-153（0.76±0.38 vs 1.92±0.95）及miR-142-3p（2.14±1.06 vs 8.15±3.20）表达水平明显低于对照组（P<0.01）。血清miR-153及miR-142-3p表达水平诊断宫颈癌的最佳截断值分别为1.31和4.93，AUC分别为0.817（95%CI：0.755～0.878）和0.850（95%CI：0.791～0.912）。血清miR-153及miR-142-3p低表达与宫颈癌患者的临床分期、分化程度、浸润深度、淋巴结转移及脉管浸润有关（P<0.05）。miR-153及miR-142-3p低表达与宫颈癌患者生存期短有关（P<0.01）。多因素Cox回归模型分析显示，临床分期、浸润深度、淋巴结转移、miR-153＜1.32及miR-142-3p＜4.93是影响宫颈癌患者预后不良的独立危险因素。［结论］ 血清miR-153及miR-142-3p表达水平在宫颈癌患者中明显下调，miR-153及miR-142-3p有望作为宫颈癌诊断及预后评估的生物学标志物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression of serum miR-153 and miR-142-3p in patients with cervical cancer and their diagnostic and prognostic value. ［Methods］ A total of 142 patients with cervical cancer admitted to our hospital from January 2014 to March 2017 were selected，and the expression levels of serum miR-153 and miR-142-3p in patients with cervical cancer were detected by real-time fluorescence quantitative PCR. ROC curve was drawn to analyze the truncation value of miR-153 and miR-142-3p in the diagnosis of cervical cancer. Based on this criterion，patients with cervical cancer were divided into high miR-153 and miR-142-3p groups and low miR-153 and miR-142-3p groups. The clinicopathological characteristics of the two groups were compared. Multivariate Cox regression models were used to analyze the risk factors affecting poor prognosis of patients with cervical cancer. ［Results］ The serum levels of miR-153（0.76±0.38 vs 1.92±0.95) and miR-142-3p（2.14±1.06 vs 8.15±3.20) in cervical cancer group were significantly lower than that in control group(P<0.01). The optimal cut-off values of serum miR-153 and miR-142-3p in the diagnosis of cervical cancer were 1.31 and 4.93，and the AUC were 0.817(95%CI：0.755~0.878) and 0.850(95%CI：0.791~0.912)，respectively. The low expression of serum miR-153 and miR-142-3p were associated with clinical stage，differentiation degree，depth of invasion，lymph node metastasis and vascular invasion of cervical cancer(P<0.05). The low expression of miR-153 and miR-142-3p were related to the short survival of patients with cervical cancer(P<0.01). Multivariate Cox regression analysis showed that clinical stage，depth of invasion，lymph node metastasis，miR-153＜1.32 and miR-142-3p＜4.93 were independent risk factors for poor prognosis of cervical cancer patients. ［Conclusion］ The expression levels of serum miR-153 and miR-142-3p are significantly down-regulated in patients with cervical cancer，and miR-153 and miR-142-3p are expected to be biomarkers for the diagnosis and prognosis of cervical cancer. |
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